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[Comparison of electrophysiological properties of vascular smooth muscle cells in different arterioles in guinea pig.] [Ariticle in Chinese]

MA Ke-Tao, LI Xin-Zhi, LI Li, ZHANG Zhi Ping, ZHAO Lei, ZHU He, SI Jun-Qiang

The Key Laboratory of Xinjiang Endemic and Ethnic Diseases； the First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi 832002, China

Abstract

Arterioles are major contributors to the control of systemic blood pressure and local blood flow. In this study, we comparedelectrophysiological properties of vascular smooth muscle cells (VSMCs) in anterior inferior cerebellar artery (AICA), mesentericartery (MA) and spiral modiolar artery (SMA) by intracellular microelectrode recording and whole-cell patch clamp recording techniques.Results were shown as below: (1) Intracellular microelectrode recordings were made from VSMCs in AICA, MA and SMA with restingpotentials of (-68±1.8) (n=65), (-71±2.4) (n=80) and (-66±2.9) mV (n=58), respectively. There was no significant difference in restingpotentials among arterioles. (2) The membrane capacitance and membrane conductance in situ cells were much larger than those indispersed smooth muscle cells by whole-cell recording techniques, and there was significant difference among arterioles, which were inthe order: MA>AICA>SMA. After application of gap junction blocker 2-APB (100 mol/L), the membrane capacitance and membraneconductance in situ cells were very close with those in single smooth muscle cells. (3) The I/V relation of whole-cell current ofdissociated smooth muscle cells (AICA, MA and SMA) showed a prominent outward rectification, and the currents were substantiallyinhibited by 1 mmol/L 4-AP or 10 mmol/L TEA. When the command voltage was +40 mV, the current densities of VSMCs in AICA, MA and SMA were (26±2.0), (24±1.7) and (18±1.3) pA/pF respectively. SMA showed significant difference in the current densityfrom AICA and MA respectively. These results suggest that the electrophysiological properties of coupling strength of gap junctionand current density of smooth muscle cells are different among arterioles in the guinea pig.

Key words: arteriole; resting membrane potential; intracellular microelectrode recording; whole-cell patch clamp recording

Received: 2010-06-03　　Accepted: 2010-08-17

Corresponding author: 司军强　　E-mail: sijunqiang11@hotmail.com

Citing This Article：

MA Ke-Tao, LI Xin-Zhi, LI Li, ZHANG Zhi Ping, ZHAO Lei, ZHU He, SI Jun-Qiang. [Comparison of electrophysiological properties of vascular smooth muscle cells in different arterioles in guinea pig.] [Ariticle in Chinese] . Acta Physiol Sin 2010; 62 (5): 421-426 (in Chinese with English abstract).