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[Change of cytochrome c in postconditioning attenuating ischemia-reperfusioninduced mucosal apoptosis in rat intestine.] [Ariticle in Chinese]

CHU Wei-Wei^*, NIE Lei, HE Xin-Ying, YAN Ai-Li, ZHOU Yi, WU Geng-Li, WANG Dian-Hua

Department of Pathology, Xi’an Medical College, Xi’an 710021, China； Yunnan Pharmacological Laboratory of Natural Products, Kunming Medical College, Kunming 650031, China

Abstract

The present study aimed to investigate the change of cytochrome c in postconditioning-attenuated ischemia-reperfusion(I/R)-induced mucosal apoptosis in rat intestine compared with ischemic preconditioning (IPC). Using rat model of intestine I/R injury,male Sprague-Dawley rats weighing 220-250 g were divided into 4 groups which were Sham operation group, I/R group, IPC group andischemic postconditioning (IPOST) group. In these groups, I/R procedure was performed by the occlusion of the superior mesentericartery (SMA) for 45 min followed by reperfusion for 1 h. In Sham group, there was no intervention. In IPC group, SMA was occludedfor 5 min and reperfused for 5 min, for two cycles, before the prolonged occlusion. In IPOST group, three cycles of 30-s reperfusionand 30-s reocclusion were preceded at the start of reperfusion. After the reperfusion, the small intestines were sampled for experimentaldetection. Intestinal mucosal mitochondrial membrane potential was detected by confocal laser scanning microscopy. Expressions ofcytochrome c and caspase-3 proteins were detected using Western-blot method. The apoptosis of intestinal mucosal cells was determinedwith agarose gel electrophoresis and deoxynucleotidyl transferase mediated dUTP-biotin nick-end labeling (TUNEL) technique.Compared with I/R group, the mitochondrial membrane potentials and the expressions of cytochrome c protein were significantlyincreased, while the expressions of caspase-3 and the apoptotic rates were decreased in IPOST and IPC groups (P<0.05). There wereno significant differences between IPOST and IPC groups (P>0.05). These data provide substantial evidence that IPOST attenuates I/R-induced mucosal apoptosis by reducing the release of cytochrome c from mitochondria in the rat small intestine.

Key words: cytochrome c; reperfusion injury; apoptosis

Received: 2009-11-16　　Accepted: 2010-01-15

Corresponding author: 褚薇薇　　E-mail: shch_ww@163.com

Citing This Article：

CHU Wei-Wei, NIE Lei, HE Xin-Ying, YAN Ai-Li, ZHOU Yi, WU Geng-Li, WANG Dian-Hua. [Change of cytochrome c in postconditioning attenuating ischemia-reperfusioninduced mucosal apoptosis in rat intestine.] [Ariticle in Chinese] . Acta Physiol Sin 2010; 62 (2): 143-148 (in Chinese with English abstract).