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[Nordihydroguaiaretic acid partially inhibits inflammatory responses after focal cerebral ischemia in rats.] [Ariticle in Chinese]

CHU Li-Sheng, FANG San-Hua, ZHOU Yu, YIN Yuan-Jun, KE Qing, CHEN Wei-Yan, WEI Er-Qing

Department of Physiology, Zhejiang Chinese Medical University, Hangzhou 310053, China； Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou 310058, China

Abstract

The aim of the present study is to investigate the role of nordihydroguaiaretic acid (NDGA) on inflammatory cells accumulationafter focal cerebral ischemia and the underlying mechanism. Focal cerebral ischemia was induced by 30 min of middle cerebralartery occlusion (MCAO) followed by 72 h of reperfusion. NDGA (5 and 10 mg/kg) was administered intraperitoneally 30 min, 2, 24,48 h after reperfusion, respectively. The brain injuries were observed by neurological and histological examination. Endogenous IgGexudation, neutrophils and macrophages/microglia accumulation, and intercellular adhesion molecule-1 (ICAM-1) protein expressionwere determined by immunohistochemistry 72 h after reperfusion. ICAM-1 mRNA was determined by RT-PCR 72 h after reperfusion.The catalysates of 5-lipoxygenase (5-LOX), leukotriene B4 (LTB4) and cysteinyl leukotrienes (CysLTs), were evaluated by ELISA 3 hafter reperfusion. The results showed that NDGA ameliorated neurological dysfunction, decreased infarct volume, and inhibitedendogenous IgG exudation, neutrophils infiltration, ICAM-1 mRNA and protein expression 72 h after reperfusion. Moreover, NDGAreduced the levels of LTB4 and CysLTs 3 h after reperfusion. However, NDGA did not reduce the accumulation of macrophages/microglia 72 h after reperfusion. These results suggest that NDGA decreases neutrophil infiltration in the subacute phase of focalcerebral ischemia via inhibiting 5-LOX activation.

Key words: nordihydroguaiaretic acid; brain ischemia; neutrophils; macrophages; microglia; 5-lipoxygenase

Received: 2009-12-07　　Accepted: 2010-01-20

Corresponding author: 储利胜　　E-mail: chulisheng@21cn.com

Citing This Article：

CHU Li-Sheng, FANG San-Hua, ZHOU Yu, YIN Yuan-Jun, KE Qing, CHEN Wei-Yan, WEI Er-Qing. [Nordihydroguaiaretic acid partially inhibits inflammatory responses after focal cerebral ischemia in rats.] [Ariticle in Chinese] . Acta Physiol Sin 2010; 62 (2): 101-108 (in Chinese with English abstract).