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The molecular mechanism of apoptosis of human umbilical veinendothelial cells induced by monocyte chemotacitic protein-1

LI Qin-Shan, LIU Yang, FENG Zan-Jie, LU Zhi-Shun, QIAN Min-Zhang

Department of Biochemistry and Molecular Biology, Zunyi Medical College, Zunyi 563003, China

Abstract

The present study was aimed to investigate whether Bcl-2, Fas and Bax are involved in monocyte chemotacitic protein-1(MCP-1)-induced apoptosis of human umbilical vein endothelial cells (hUVECs). hUVECs were cultured, and the purity was identifiedby immunofluorescence and immunohistochemistry with specific anti-von Willebrand factor (vWF) and anti-VEGF receptor-2 (KDR)antibodies. With 90% confluence hUVECs were serum-starved for 12 h, and then treated with different concentrations of MCP-1(0.1, 1.0, 10, 100 ng/mL) for 24 and 48 h respectively. The expressions of apoptosis related proteins Fas, Bcl-2, Bax were detected byflow cytometry (FACS) and Western blot. As shown in our preliminary study, MCP-1 induced apoptosis of hUVECs in a dosedependentmanner at both 24 h and 48 h. FACS and Western blot analysis results in the present study indicated that MCP-1 promotedthe expression of proapoptotic proteins Bax and Fas and inhibited the expression of antiapoptotic protein Bcl-2. These results suggestthat MCP-1 may induce the apoptosis of hUVECs through evoking the imbalance between proapoptotic Fas/Bax and antiapoptoticBcl-2 protein.

Key words: monocyte chemotacitic protein-1; human umbilical vein endothelial cell; atherosclerosis; Bcl-2; Bax; Fas; apoptosis

Received: 2009-09-03　　Accepted: 2010-01-04

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Citing This Article：

LI Qin-Shan, LIU Yang, FENG Zan-Jie, LU Zhi-Shun, QIAN Min-Zhang. The molecular mechanism of apoptosis of human umbilical veinendothelial cells induced by monocyte chemotacitic protein-1. Acta Physiol Sin 2010; 62 (1): 63-68 (in Chinese with English abstract).