Issue Archive

[MG-132 enhances MSCs survival and IL-10 secretion under hypoxia and serum deprivation condition.] [Article in Chinese]

LI Zong-Wei, FU Rong, ZHAO Ya-Rui, ZHAO Chao, LI Zhuo-Yu^*

Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education, Shanxi University, Taiyuan 030006, China

Abstract

Bone marrow-derived mesenchymal stem cells (MSCs) have emerged as attractive candidates for cellular therapies for heart and other organ-system disorders. However, a major dilemma in stem cell therapy for ischemic heart diseases is the low survival of transplanted cells in the ischemic and peri-infarcted region. In this study, MSCs were treated by hypoxia and serum deprivation (H/SD) to mimic the ischemic microenvironment of infarcted hearts where MSCs were transplanted. The effects of proteasome inhibitor MG-132 on H/SD-induced apoptosis and paracrine effects were investigated. Apoptosis of MSCs was detected by Annexin V-FITC flow cytometric analysis. Transcriptional levels of IL-1β, TNF-α and IL-10 were examined by real-time PCR. The nuclear translocation of NF-κBp65 was assessed by immunocytochemical staining. Translational changes of IL-1β and TNF-α were detected by Western blot. The secretion of IL-10 from MSCs was examined by ELISA assay. The results showed that MG-132 could effectively suppress H/SD-induced MSCs apoptosis. Furthermore, the induced IL-1β and TNF-α transcription was also inhibited by MG-132 treatment, which may be due to the inhibition of NF-κBp65 nuclear translocation by MG-132. Importantly, MG-132 effectively enhanced H/SD-induced transcription and secretion of IL-10, which is an important paracrine factor from MSCs. Our findings suggest that pretreatment of MSCs by MG-132 before cell transplantation may be an effective strategy to improve cell survival and enhance paracrine effects of MSCs.

Key words: MG-132; mesenchymal stem cell; IL-10; apoptosis; paracrine

Received: 2011-04-06　　Accepted: 2011-07-05

Corresponding author: 李卓玉　　E-mail: lzy@sxu.edu.cn

Citing This Article：

LI Zong-Wei, FU Rong, ZHAO Ya-Rui, ZHAO Chao, LI Zhuo-Yu. [MG-132 enhances MSCs survival and IL-10 secretion under hypoxia and serum deprivation condition.] [Article in Chinese]. Acta Physiol Sin 2011; 63 (6): 525-532 (in Chinese with English abstract).