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[Suppressing poly(ADP-ribose)polymerase-1 inhibits tau phosphorylation in HEK293/tau441 cells.] [Article in Chinese]

CHEN Qin-Hao, LIAO Xiao-Mei, WANG Shao-Hui^*

College of Life Science, Huazhong Normal University, Wuhan 430079, China

Abstract

The study aimed to investigate the effect of inhibition of poly(ADP-ribose) polymerase-1 (PARP-1) activity on tau phosphorylation in HEK293/tau441 cells and its mechanism. HEK293/tau441 cells were treated with 3-aminobenzamide (3-AB), a PARP-1 inhibitor, at different doses (0.5, 1, 2, 4 mmol/L). After 24 h, the cell morphology was observed under phase contrast microscope, tau phosphorylation level in different sites (tau-1, tau-5, Thr231) and the activity of glycogen synthase kinase 3 (GSK-3) were detected by Western blotting. The results showed: (1) 3-AB at different doses failed to change the morphology of cells; (2) The 3-AB-induced decrease in activity of PARP-1 resulted in increase of unphosphorylation level in tau-1(Ser195/198/199/202) sites; (3) The phosphorylation of tau was decreased in Thr231 site, while the total tau was slightly changed after 3-AB treatment; (4) With the increased phosphorylation of GSK-3 at Ser9 site, the activity of GSK-3 was decreased after 3-AB treatment. The results suggest that the inhibition of PARP-1 by 3-AB could decrease tau phosphorylation in HEK293/tau441 cells probably through decreasing GSK-3 activity.

Key words: poly(ADP-ribose)polymerase-1; glycogen synthase kinase 3; Tau protein; Alzheimer's disease

Received: 2011-04-21　　Accepted: 2011-06-16

Corresponding author: 王少辉　　E-mail: wsh2002505@163.com

Citing This Article：

CHEN Qin-Hao, LIAO Xiao-Mei, WANG Shao-Hui. [Suppressing poly(ADP-ribose)polymerase-1 inhibits tau phosphorylation in HEK293/tau441 cells.] [Article in Chinese]. Acta Physiol Sin 2011; 63 (6): 511-516 (in Chinese with English abstract).