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[Involvement of protein kinase C in enhancement of vascular calcium sensitivity by blocking mesenteric lymph return in hemorrhagic shock rats.] [Article in Chinese]

NIU Chun-Yu^*, ZHAO Zi-Gang, WEI Yan-Ling, ZHANG Yu-Ping, ZHANG Jing

Institute of Microcirculation, Department of Pathophysiology of Basic Medical Institute, Hebei North University, Zhangjiakou 075000, China

Abstract

The aim of the present study was to investigate whether protein kinase C (PKC) was involved in the effect of mesenteric lymph duct ligation or mesenteric lymph drainage on vascular calcium sensitivity in hemorrhagic shock rats. Male Wistar rats were randomly divided into Sham, Shock (hemorrhagic shock), Shock+Ligation (mesenteric lymph duct ligation plus shock) and Shock+Drainage (mesenteric lymph drainage plus shock) groups. After being in shock (hypotension 40 mmHg) for 3 h, the tissue of superior mesenteric artery (SMA) was taken out for detecting the PKC expression and phospho-PKC (p-PKC) activity, and the vascular rings of SMA were prepared and used to measure the response to gradient calcium concentration for assaying the calcium sensitivity, the parameters of which including tension, maximum tension (Emax) and negative logarithm of EC50, called the pD2. Other vascular rings from Shock+Ligation and Shock+Drainage groups were incubated with PKC regulator PMA or Staurosporine before the measurement of calcium sensitivity. The results showed that, PKC expression, p-PKC activity and calcium sensitivity of SMA in Shock group was significantly lower than that of Sham group, whereas the above-mentioned indexes were significantly elevated in Shock+Ligation and Shock+Drainage groups compared with those in Shock group. PKC agonist PMA enhanced the contractile activity of vascular rings to gradient calcium ions, and increased Emax of SMA in Shock+Ligation and Shock+Drainage groups. On the contrary, PKC inhibitor Staurosporine significantly decreased the response to gradient calcium ions and Emax of SMA in Shock+Ligation and Shock+Drainage groups. These results suggest that PKC plays a role in the improvement of vascular calcium sensitivity by blockade of mesenteric lymph return in hemorrhagic shock rats.

Key words: Hemorrhagic shock; calcium sensitivity; protein kinase C; mesenteric lymph duct; ligation; mesenteric lymph; drainage

Received: 2011-08-05　　Accepted: 2011-12-06

Corresponding author: 牛春雨　　E-mail: ncylxf@126.com

Citing This Article：

NIU Chun-Yu, ZHAO Zi-Gang, WEI Yan-Ling, ZHANG Yu-Ping, ZHANG Jing. [Involvement of protein kinase C in enhancement of vascular calcium sensitivity by blocking mesenteric lymph return in hemorrhagic shock rats.] [Article in Chinese]. Acta Physiol Sin 2012; 64 (2): 213-219 (in Chinese with English abstract).