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[β-estradiol activates BK(Ca) in mesenteric artery smooth muscle cells of post-menopause women.] [Article in Chinese]

Cheng Jun, ZENG Xiao-Rong, LI Peng-Yun, LU Ting-Ting, TAN Xiao-Qiu, WEN Jing, YANG Yan^*

Department of Electrophysiology, Institute of Cardiovasology, State Key Laboratory, Luzhou Medical College, Luzhou 646000, China

Abstract

The aim of the present study was to study the effect of β-estradiol (β-E2) on the large-conductance Ca2+-activated potassium (BKCa) channel in mesenteric artery smooth muscle cells (SMCs). The mesenteric arteries were obtained from post-menopause female patients with abdominal surgery, and the SMCs were isolated from the arteries using an enzymatic disassociation. According to the sources, the SMCs were divided into non-hypertension (NH) and essential hypertension (EH) groups. Single channel patch clamp technique was used to investigate the effect of β-E2 and ICI 182780 (a specific blocker of estrogen receptor) on BKCa in the SMCs. The results showed the opening of BKCa in the SMCs was voltage and calcium dependent, and could be blocked by IbTX. β-E2 (100 μmol/L) significantly increased open probability (Po) of BKCa in both NH and EH groups. After β-E2 treatment, NH group showed higher Po of BKCa compared with EH group. ICI 182780 could inhibit the activating effect of β-E2 on BKCa in no matter NH or EH groups. These results suggest β-E2 activates BKCa in mesenteric artery SMCs from post-menopause women via estrogen receptor, but hypertension may decline the activating effect of β-E2 on BKCa.

Key words: Large-conductance calcium-activated potassium channel; β-estradiol; estrogen receptor; human mesenteric artery smooth muscle; hypertension

Received: 2011-10-18　　Accepted: 2011-12-29

Corresponding author: 杨艳　　E-mail: wyangyan@gmail.com

Citing This Article：

Cheng Jun, ZENG Xiao-Rong, LI Peng-Yun, LU Ting-Ting, TAN Xiao-Qiu, WEN Jing, YANG Yan. [β-estradiol activates BK(Ca) in mesenteric artery smooth muscle cells of post-menopause women.] [Article in Chinese]. Acta Physiol Sin 2012; 64 (2): 121-128 (in Chinese with English abstract).