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[Adiponectin up-regulates the expression of T-cadherin in cardiomyocytes injured by hypoxia/reoxygenation.] [Article in Chinese]

Wang Min, CHAI Ying-Ru, XIAO Chuan-Shi, ZHAO Xu-Jing, WEI Na, BAI Rui, BIAN Yun-Fei^*

Department of Cardiology, the Second Affiliated Hospital of Shanxi Medical University, Taiyuan 030001, China

Abstract

The aim of the present study was to investigate the effects of adiponectin (APN) on the expression of T-cadherin in cultured Sprague-Dawley (SD) rat cardiomyocytes injured by hypoxia/reoxygenation (H/R). Primary myocardial cells from neonatal rats were obtained by enzymatic digestion. The cells were divided into control group, H/R group and H/R+APN (3, 10, 20 and 30 μg/mL) groups. The H/R group was incubated in anoxic environment (anoxic solution saturated with high concentration N2) for 3 h, and then in the reoxygenation environment (the reoxygenation solution saturated with pure oxygen) for 1 h. The H/R+APN group was pretreated with different concentrations of APN for 24 h prior to the initiation of H/R. The content of lactate dehydrogenase (LDH) was measured by chemistry chromatometry. Cellular apoptosis was analyzed by flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). The expression of T-cadherin was detected by RT-PCR and Western blotting. The results showed that, compared with control group, the apoptotic rate and release of LDH were significantly increased in the H/R group, whereas the expressions of T-cad mRNA and protein were decreased. Pretreating with APN significantly and dose-dependently decreased apoptotic rate and LDH release, and up-regulated T-cad mRNA and protein level in rat neonatal cardiomyocytes under H/R conditions. These results suggest that APN may protect cardiomyocytes against H/R-induced injury by up-regulating H/R-decreased T-cad expression.

Key words: adiponectin; cardiomyocytes; hypoxia/reoxygenation; T-cadherin

Received: 2011-12-06　　Accepted: 2012-04-26

Corresponding author: 边云飞　　E-mail:

Citing This Article：

Wang Min, CHAI Ying-Ru, XIAO Chuan-Shi, ZHAO Xu-Jing, WEI Na, BAI Rui, BIAN Yun-Fei. [Adiponectin up-regulates the expression of T-cadherin in cardiomyocytes injured by hypoxia/reoxygenation.] [Article in Chinese]. Acta Physiol Sin 2012; 64 (3): 296-302 (in Chinese with English abstract).