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[Protective effects of sodium butyrate against lung injury in mice with endotoxemia.] [Article in Chinese]

ZENG Tao, ZHANG Li

Medical College, Jingchu University of Technology, Jingmen 448000, China； Department of Pathophysiology, Chongqing Medical University, Chongqing 400016, China

Abstract

The aim of the present study was to investigate the effects of sodium butyrate (SB) on systemic inflammation, lung injury and survival rate of mice with endotoxemia. Balb/c mice were pre-treated with SB or vehicle, and then endotoxemia was induced by lethal dose of lipopolysaccharide (LPS, 20 mg/kg, i.p.) and the survival rate of mice was monitored. A separated set of animals were sacrificed at 18 h after LPS challenge, and blood samples were harvested for measuring TNF-α and IL-6 levels. Lung tissues were also harvested to determine the ratio of wet weight to dry weight of lung tissue and myeloperoxidase (MPO) activity in lung tissue. In addition, the formalin-fixed lung specimens were stained with HE routinely for morphologic evaluation. The results showed that pre-treatment with SB alleviated LPS-induced morphological damage in lung tissue. This was accompanied by reduced ratio of wet weight to dry weight of lung tissue and MPO activity in lung homogenates. Additionally, the up-regulation of pro-inflammatory cytokines TNF-α and IL-6 was also suppressed by SB, while the survival rate of mice with lethal endotoxemia was significantly increased by SB pre-treatment. The results suggest that SB effectively attenuates intrapulmonary inflammatory response and improves the survival of endotoxemic mice.

Key words: histone deacetylase; endotoxemia; lung injury; inflammation

Received: 2011-11-06　　Accepted: 2012-01-05

Corresponding author: 张力　　E-mail: zhangli@cqmu.edu.cn

Citing This Article：

ZENG Tao, ZHANG Li. [Protective effects of sodium butyrate against lung injury in mice with endotoxemia.] [Article in Chinese]. Acta Physiol Sin 2012; 64 (3): 308-312 (in Chinese with English abstract).