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[Hydrogen sulfide reduces lipopolysaccharide-induced acute lung injury and Hydrogen sulfide reduces lipopolysaccharide-induced acute lung injury and inhibits expression of phosphorylated p38 MAPK in rats.] [Article in Chinese]

FAN YaMin, HUANG XinLi^*, DONG ZeFei, LING YiLing

Departments of Ophthalmology, Physiology, Xingtai Medical College, Xingtai 054000, China； Department of Pathophysiology, Hebei Medical University, Shijiazhuang 050017, China

Abstract

To investigate the influence of hydrogen sulfide (H2S) on p38 MAPK signaling pathway during acute lung injury (ALI) caused by lipopolysaccharide (LPS), the rats were randomly divided into six groups: control group, LPS group, LPS + NaHS group, LPS + PPG (cystathionine-γ-lyase inhibitor) group, NaHS group and PPG group. The rats were sacrificed 6 h after injection and lung tissues were obtained. The structure of lung tissues and the number of polymorphonuclear leucocyte (PMN) was observed under optical microscope; the lung myeloperoxidase (MPO) activity, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were tested; intercellular adhesion molecule-1 (ICAM-1) protein expression changes were detected by immunohistochemical staining; phosphorylated p38 MAPK (p-p38 MAPK) protein expression was detected by Western blotting. The results showed that the lung injury in LPS group was observed, at the same time the MPO activity, the content of MDA, ICAM-1 and p-p38 MAPK protein expressions, the number of PMN were all higher than those in control group (all P < 0.05). Pre-injection of NaHS alleviated the changes induced by LPS, while pre-injection of PPG aggravated those alterations (all P < 0.05). ICAM-1 and p-p38 MAPK protein expressions in lung tissue were positively correlated (r = 0.923, P < 0.01). The results suggest that H2S may reduce LPS-induced ALI through inhibiting the conjugation of p38 MAPK and reducing the expression of ICAM-1.

Key words: hydrogen sulfide; acute lung injury; intercellular adhesion molecule-1; p38 MAPK

Received: 2012-03-05　　Accepted: 2012-08-02

Corresponding author: 黄新莉　　E-mail: huangxinli2006@hotmail.com

Citing This Article：

FAN YaMin, HUANG XinLi, DONG ZeFei, LING YiLing. [Hydrogen sulfide reduces lipopolysaccharide-induced acute lung injury and Hydrogen sulfide reduces lipopolysaccharide-induced acute lung injury and inhibits expression of phosphorylated p38 MAPK in rats.] [Article in Chinese]. Acta Physiol Sin 2012; 64 (6): 666-672 (in Chinese with English abstract).