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[Inhibitory effects of soluble endoglin on invasive ability of cultured cytotrophoblasts of first trimester of pregnancy.] [Article in Chinese]

DONG Wei, XU Qun-Xing, HAN Yu-Huan, YIN Li-Rong

Department of Obstetrics, the Second Hospital of Tianjin Medical University, Tianjin 300211, China

Abstract

The present study aims to investigate the effects of soluble endoglin (sEng) on invasive ability of cultured cytotrophoblasts of first trimester of pregnancy. Cytotrophoblasts of normal 6 to 8-week pregnancy were cultured by trypsin digestion method, and were incubated with cell culture medium without (control group) and with 10 μg/L sEng (sEng group), respectively for 24 h. The invasive ability was determined by transwell invasion assay, and expressions of MMP-2, MMP-9 mRNA and protein were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot, respectively. The results showed that the invasive ability of cytotrophoblasts in sEng group was lower than that in control group (P < 0.05). Compared with control group, the expressions of MMP-2 and MMP-9 mRNA and protein of cytotrophoblasts were significantly lower (P < 0.05). In conclusion, sEng may participate in the genesis of preeclampsia by affecting the invasive ability of cytotrophoblasts through regulation of the expression of MMP-2 and MMP-9.

Key words: preeclampsia; cytotrophoblast; matrix metalloproteinases

Received: 2010-12-17　　Accepted: 2011-03-14

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Citing This Article：

DONG Wei, XU Qun-Xing, HAN Yu-Huan, YIN Li-Rong . [Inhibitory effects of soluble endoglin on invasive ability of cultured cytotrophoblasts of first trimester of pregnancy.] [Article in Chinese]. Acta Physiol Sin 2011; 63 (3): 267-271 (in Chinese with English abstract).