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[c-SRC knockdown decreases phosphorylated STAT3 expression and viability of HeLa cells.] [Article in Chinese]

CHEN Jia-Xiang, XU Lin-Lin, WU Sheng-Jiao, LIU Hong-Yu, WANG Jing-Lei^*, ZOU Ting

Department of Physiology, Medical College； Medical Research Center, the First Affiliated Hospital, Nanchang University, Nanchang 330006, China

Abstract

The present study was to determine the effect of c-SRC on the viability of human cervical cancer HeLa cells and the expression of phosphorylated signal transducer and activator of transcription-3 (p-STAT3) of the cell. Post-transfection of c-SRC RNA interference vector, RT-PCR and Western blot were utilized to observe the contents of c-SRC mRNA and protein, respectively, in HeLa cells. The MTT was used to observe the viability of the cells. Cell cycle was observed by flow cytometry. The content of p-STAT3 in the cells was also investigated after knockdown of c-SRC. Knockdown of c-SRC significantly decreased the contents of c-SRC mRNA and protein in the cells. The viability of the cells decreased by 23.1%, 29.3%, 38.6% and 45.0% (all P < 0.05), respectively, after the cells were transfected with c-SRC RNA interference vector for 24, 48, 72, and 96 h. The number of S-phase cells decreased by 5.6%, 10.0%, 15.2% and 19.9% (all P < 0.05), respectively, after transfection of c-SRC RNA interference vector for 24, 48, 72, and 96 h. The content of p-STAT3 also decreased when c-SRC was knockdowned. Compared with the control group, after treatment of HeLa cells with STAT3 inhibitor Piceatannol for 24, 48, 72, and 96 h, the cell viability decreased by 23.8%, 29.7%, 37.3% and 45.4% (all P < 0.05), respectively, while increase of c-SRC content could not reverse the inhibitory effect. These results suggest that the inhibited viability of HeLa cells caused by knockdown of c-SRC is associated with the decreased content of p-STAT3 protein.

Key words: cervical cancer; c-SRC; signal transducer and activator of transcription-3

Received: 2010-12-06　　Accepted: 2011-03-25

Corresponding author: 王晶磊　　E-mail: wangjinglei62@163.com

Citing This Article：

CHEN Jia-Xiang, XU Lin-Lin, WU Sheng-Jiao, LIU Hong-Yu, WANG Jing-Lei, ZOU Ting. [c-SRC knockdown decreases phosphorylated STAT3 expression and viability of HeLa cells.] [Article in Chinese]. Acta Physiol Sin 2011; 63 (3): 198-204 (in Chinese with English abstract).