Issue Archive

[Gossypol acetic acid induces DNA double-strand breaks in human mucoepidermoid carcinoma cell MEC-1.] [Article in Chinese]

Guo Zhong, ZHAO Jin^*, XUE Tong-Min, MA Jian-Xiu, WANG Chen-Jing, HUANG Shuang-Sheng

Medical College of Northwest University for Nationalities, Lanzhou 730030, China

Abstract

The present study was conducted to investigate the effects of gossypol acetic acid (GAA) on the proliferation of human mucoepidermoid carcinoma cell line MEC-1 in vitro and its possible molecular mechanisms of DNA double-strand breaks (DSB). MTT assay was performed to test the inhibition of proliferation of MEC-1 cells by GAA. DSB and γH2AX foci formation induced by GAA were detected by neutral comet assay and immunostaining. GAA (5–40 μmol/L) inhibited the growth of MEC-1 cells in a dose- and time-dependent manner. One of the indexes of comet assay, percentage of head DNA was decreased, however other indexes, including tail length, percentage of tail DNA, tail moment (TM) and Olive tail moment (OTM) were increased when treated with 2.5– 40 μmol/L GAA for 24 h or 20 μmol/L GAA for 3–48 h, compared with those in control. The percentage of γH2AX-positive cells was also increased when MEC-1 was treated with 2.5–20 μmol/L GAA for 24 h or 20 μmol/L GAA for 3–48 h, compared with that in control. All these results show that GAA inhibits the proliferation of MEC-1, and DSB maybe one of the mechanisms of inhibitory effect of GAA on the growth of tumor cells.

Key words: gossypol acetic acid; human mucoepidermoid carcinoma cell-1; double-strand breaks; γH2AX

Received: 2010-10-27　　Accepted: 2011-01-07

Corresponding author: 赵晋　　E-mail: yxzj@xbmu.edu.cn

Citing This Article：

Guo Zhong, ZHAO Jin, XUE Tong-Min, MA Jian-Xiu, WANG Chen-Jing, HUANG Shuang-Sheng. [Gossypol acetic acid induces DNA double-strand breaks in human mucoepidermoid carcinoma cell MEC-1.] [Article in Chinese]. Acta Physiol Sin 2011; 63 (2): 164-170 (in Chinese with English abstract).