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Polydatin attenuates ischemia/reperfusion-induced apoptosis in myocardium of the rat

ZHANG Li-Ping, MA Hui-Jie, BU Hui-Min, WANG Mei-Ling, LI Qian, QI Zhao, ZHANG Yi^*

Department of Physiology, Hebei Medical University, Shijiazhuang 050017, China

Abstract

The aim of the present study was to investigate the effect of polydatin on apoptosis induced by ischemia/reperfusion (I/R) in rat myocardium and to explore the underlying mechanism. Adult male Sprague-Dawley (SD) rats were randomly divided into control, I/R and polydatin (50 μmol/L) groups. On the Langendorff apparatus, isolated rat heart was subjected to 30-min global ischemia followed by 60-min reperfusion. TUNEL labeling and flow cytometric techniques were used for the measurement of apoptosis and the expression of Bcl-2 and Bax protein in cardiomyocytes of rat. The results showed: (1) Compared with those in the control group, the number of TUNEL-positive cells and apoptosis rate were increased in I/R group; (2) Compared with that in the I/R group, the number of TUNEL-positive cells was significantly decreased in the polydatin group [(18.1±4.0)% vs (35.1±5.4)%, P<0.01]; (3) Apoptosis rate assayed by flow cytometry in I/R group was significantly higher than that in polydatin group [(15.43±4.55)% vs (8.66±3.18)%, P<0.01]; (4) Expression level of Bax protein was higher in I/R group than that in polydatin group (P<0.05), while the level of Bcl-2 protein and Bcl-2/Bax ratio were higher in polydatin group than those in I/R group (P<0.05, P<0.01), respectively. The results obtained suggest that polydatin exerts an inhibitory effect on I/R-induced apoptosis through increasing Bcl-2 protein expression and decreasing Bax protein expression in myocardium of the rat.

Key words: polydatin; apoptosis; ischemia/reperfusion; heart; rat

Received: 2009-04-23　　Accepted: 2009-06-25

Corresponding author: 张翼　　E-mail: zhyhenry@hotmail.com

Citing This Article：

ZHANG Li-Ping, MA Hui-Jie, BU Hui-Min, WANG Mei-Ling, LI Qian, QI Zhao, ZHANG Yi. Polydatin attenuates ischemia/reperfusion-induced apoptosis in myocardium of the rat. Acta Physiol Sin 2009; 61 (4): 367-372 (in Chinese with English abstract).