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[Involvement of p38 MAPK pathway in GLP-1-induced inhibition of apoptosis in human umbilical vein endothelial cells.] [Article in Chinese]

XU Hua, LI Hai-Long, NIU Zi-Yong, LI Gui-Zhong, CAO Jun, JIANG Yi-Deng

Department of Pathophysiology, Ningxia Medical University, Yinchuan 750004； Department of Internal Medicine, Ningxia Third Hospital, Yinchuan 750021

Abstract

The aim of the present study was to investigate the effect of glucagon-like peptide-1 (GLP-1) on palmitate-induced apoptosis of human umbilical vein endothelial cells (HUVECs) and the underlying mechanism. HUVECs were cultured in vitro, and then treated by palmitate to induce apoptosis. Meanwhile, GLP-1 was added to explore its effect. After 24 h of the treatments, Caspase-3 activity and DNA fragmentation were measured using ELISA kits. Phospho-p38 mitogen-activated protein kinase (p-p38 MAPK) expression was detected by Western blot. The results showed that incubating HUVECs with 0.125 mmol/L GLP-1 increased Caspase-3 activity and DNA fragmentation. GLP-1 significantly inhibited palmitate-induced increases of Caspase-3 activity and DNA fragmentation in a concentration-dependent manner. Moreover, GLP-1 inhibited the up-regulation of p-p38 MAPK expression induced by palmitate in HUVECs. These results suggest GLP-1 protects HUVECs against lipo-apoptosis, and this effect may be mediated through inhibiting p38 MAPK pathway.

Key words: glucagon-like peptide-1; human umbilical vein endothelial cells; p38 MAPK; apoptosis

Received: 2012-02-28　　Accepted: 2012-05-28

Corresponding author: 曹军,姜怡邓　　E-mail: jwcjyd@163.com, CAO Jun: Tel: +86-951-6980093

Citing This Article：

XU Hua, LI Hai-Long, NIU Zi-Yong, LI Gui-Zhong, CAO Jun, JIANG Yi-Deng. [Involvement of p38 MAPK pathway in GLP-1-induced inhibition of apoptosis in human umbilical vein endothelial cells.] [Article in Chinese]. Acta Physiol Sin 2012; 64 (4): 444-448 (in Chinese with English abstract).