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[The protective mechanism of N-acetylcysteine against ischemia/reperfusioninduced gastric injury in rats.] [Ariticle in Chinese]

ZHOU Xiao-Yan, DU Dong-Shu, MA Xiao-Bo, ZHANG Jian-Fu

Department of Physiology； Laboratory of Morphological Experiments, Xuzhou Medical College, Xuzhou 221002, China

Abstract

The present study aimed to investigate the protective mechanism of N-acetylcysteine (NAC) against gastric ischemia /reperfusion (GI/R) injury in rats. After intravenous injection (IV) of NAC (150 mg/kg) into femoral vein, the rats were subjected to 30min of ischemia induced by clamping the celiac artery followed by 60 min of reperfusion. After the gastric mucosal damage index(GMDI) had been calculated, gastric mucosal cell in situ apoptosis was detected by TUNEL method. The protein expression of p-ERK,p-JNK and NF-κB, and mRNA expression of TNF-α and Caspase-3 in gastric mucosa were evaluated by using Western-blot or RTPCR,respectively. The results showed that NAC not only attenuated the GI-R injury, but also decreased gastric mucosal cellularapoptosis. Furthermore, NAC increased the protein expression of p-ERK, while inhibited protein expression of p-JNK, NF-κB ingastric mucosa. NAC also decreased the expression of TNF-α mRNA and Caspase-3 mRNA in gastric mucosa. Capsazepine (CPZ)(400 mg/kg, IV) reversed the protective effect of NAC against GI/R injury in rats. These results suggest that NAC can protect ratsagainst GI/R injury. This protective effect is possibly mediated by the up-regulation of p-ERK and down-regulation of p-JNK and NF-κB.In addition, vanilloid receptor subtype 1 may be involved in the protective mechanism of NAC against GI/R injury.

Key words: N-acetylcysteine; gastric ischemia/reperfusion; apoptosis; vanilloid receptor subtype 1; rats

Received: 2009-09-30　　Accepted: 2009-11-26

Corresponding author: 张建福　　E-mail: jfzhang@xzmc.edu.cn

Citing This Article：

ZHOU Xiao-Yan, DU Dong-Shu, MA Xiao-Bo, ZHANG Jian-Fu. [The protective mechanism of N-acetylcysteine against ischemia/reperfusioninduced gastric injury in rats.] [Ariticle in Chinese] . Acta Physiol Sin 2010; 62 (1): 69-72 (in Chinese with English abstract).