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Overexpression of PDZ1 domain of PSD--95 protein rescues hippocampl neurons from apoptosis induced by oxygen--glucose deprivation

Hu Shuqun, Zong Yanyan, Zhang Guangyi

Research Center of Biochemistry and Molecular Biology；China

Abstract

To detect the effect of PDZ1,domain of postsynaptic density 95(PSD-95),on apoptosis of hippocampal neurons induced by oxygen-glucose deprivation(OGD),Sprague-Dawley rat hippocampal neurons were infected with PDZ1-viruses after 21 days of plating.Twenty-four hours after infection,cells were treated with OGD for 1.5 h,then were incubated with DAPI and apoptosis-like cells were characterized,or were collected for co-immunoprecipitation and Western blot analyses.The results showed that:(1) PDZ1 overexpression was observed in hippocampal neurons;(2) Apoptosis induced by OGD was obviously decreased in neurons overexpressing PDZ1(P<0.05);(3) Overexpression of PDZ1 prevented the binding of GluR6 to PSD-95;(4) Overexpression of PDZ1 inhibited MLK3 and JNK1/2 activation induced by OGD.These results indicate that overexpression of PDZ1 may prevent hippocam-pal neurons from apoptosis induced by OGD.

Key words: PDZ1;oxygen-glucose deprivation;Hippocampal neurons;apoptosis

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Citing This Article：

Hu Shuqun, Zong Yanyan, Zhang Guangyi. Overexpression of PDZ1 domain of PSD--95 protein rescues hippocampl neurons from apoptosis induced by oxygen--glucose deprivation. Acta Physiol Sin 2008; 60 (6): 730-736 (in Chinese with English abstract).