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[H2O2 promotes neutrophil adherence and injury of human umbilical vein endothelial cells.] [Article in Chinese]

QIAN Zhong-Qing^*, HE Xiao-Yan, LV Jing-Zhu, WANG Hong-Tao, FANG Fang

Key Laboratory of Anhui Province for Infection and Immunology, Department of Immunology, Bengbu Medical College, Bengbu 233003, China

Abstract

To explore the role of hydrogen peroxide (H2O2) in promoting polymorphonuclear neutrophils adherence and injury of human umbilical vein endothelial cells (HUVECs), the ordinary optical microscope and scanning electron microscopy were used to observe the adherence and injury after HUVECs co-cultured with neutrophils pretreated by extracellular H2O2 (HUVECs and neutrophils co-culture without H2O2 pretreatment as control), and the adhesion rates of neutrophils were measured through cell count test. The percentages of HUVECs expressing intercellular adhesion molecule 1 (ICAM-1) and Apo2.7 were detected by flow cytometry. After being cocultured with the neutrophils pretreated by extracellular H2O2, HUVECs showed obvious injury changes, such as round or oval shape, shortened or disappeared microvilli, and membrane structural damage; The adhesion rate of neutrophils was (57.74 ± 9.18)%, which was significantly higher than that in control [(23.12 ± 6.43)%, P < 0.01, n = 8]; The percentages of HUVECs expressing ICAM-1 and Apo2.7 were (44.69 ± 1.52)% and (39.29 ± 1.81)% respectively, which were significantly higher than those in control [(21.79 ± 1.43)% and (9.79 ± 1.43)%] (P < 0.01, n = 8). The results suggest that extracellular H2O2 can promote the neutrophils adherence and injury of HUVECs.

Key words: Hydrogen peroxide; neutrophil; human umbilical vein endothelial cell; intercellular adhesion molecule 1

Received: 2012-01-05　　Accepted: 2012-04-20

Corresponding author: 钱中清　　E-mail: qzq7778@yahoo.com.cn

Citing This Article：

QIAN Zhong-Qing, HE Xiao-Yan, LV Jing-Zhu, WANG Hong-Tao, FANG Fang. [H2O2 promotes neutrophil adherence and injury of human umbilical vein endothelial cells.] [Article in Chinese] . Acta Physiol Sin 2012; 64 (4): 412-416 (in Chinese with English abstract).