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Effects of GDP on the activity and expression of mitochondrial uncoupling proteins in rat brain {sl in vitro}

Xia Chen, Liu Junze, Xu Yu

Department of Pathophysiology,College of High Altitude Military Medicine,the Third Military Medical University.Chongqing 400038

Abstract

To investigate the regulatory role of purine nucleotide on uncoupling proteins (UCPs), the activity of UCPs and the expressions of UCP4 and UCP5 in mitochondria of rat brain tissues incubated with GDP were observed in vitro. The cerebral hemispheres of adult male Sprague-Dawley rats were removed and clipped into 8-10 mm~(3) tissue mass which incubated with 1 mmol/L GDP (GDP group), or only incubation medium (control group), for 30 min in vitro. The mitochondria from incubated tissue mass of rat brain were isolated by centrifugation. The activity of UCPs was detected by the method of [~(3)H]-GTP binding with UCPs specifically. The maximal binding content (B_(max)) and the dissociation constant (K_(d)) were determined from Scatchard plot. The mRNA and protein expressions of UCP4 and UCP5 were measured by RT-PCR and Western blot, respectively. The results showed that B_(max) was increased and K_(d) was decreased in rat brain mitochondria in GDP group compared with that in control group. But the mRNA and protein expressions of UCP4 and UCP5 exhibited no statistically significant changes. It is thus suggested that GDP inhibits the activity of mitochondrial UCPs in rat brain in vitro, but exhibits no effect on the expressions of UCP4 and UCP5.

Key words: GDP;Brain;uncoupling protein

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Citing This Article：

Xia Chen, Liu Junze, Xu Yu. Effects of GDP on the activity and expression of mitochondrial uncoupling proteins in rat brain {sl in vitro}. Acta Physiol Sin 2008; 60 (4): 492-496 (in Chinese with English abstract).