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Differential expressions of nNOS and iNOS in the rostral ventrolateral medulla induced by electroacupuncture in acute myocardial ischemia rats

Xia Chunmei, Chen Jun, Wang Jin, Fan Mingxin, Xiao Fen, Cao Yinxiang, Li Li, Shen Linlin, Zhu Danian

Department of Physiology and Pathophysiology,Shanghai Medical College of Fudan University.Shanghai 200032

Abstract

Increasing lines of evidence has been accumulated that nitric oxide (NO) and nitric oxide synthase (NOS) distribute plentifully in the rostral ventrolateral medulla (RVLM) and contribute to cardiovascular regulation. In the present study, the expressions of neuronal and inducible isoform of NOS (nNOS and iNOS) were observed in the RVLM of acute myocardial ischemia (AMI) Wistar rats experienced electroacupuncture (EA) treatment, thereby the cardiovascular effects of NO in the RVLM were investigated and the mechanism of acupuncture effect on AMI was inferred. The results indicated that in the AMI rats, cardiac functions were markedly attenuated with high serum level of brain natriuretic peptide (BNP) and norepinephine (NE), the number of nNOS-immunoreactive cells and nNOS mRNA exprossion in the RVLM area were increased, while those of iNOS were lowered. EA at "Neiguan" acupoints (Pe 6) 30 min daily for successive 5 d resulted in an improvement of the cardiac functions, decreases in NE and BNP levels; it also increased the expression of iNOS and decreased the expression of nNOS in the RVLM. These results suggest that the curative effect of acupuncture on AMI is possibly attributable to the differential regulation of NOS/NO in the RVLM, leading to decreased sympathetic outflow and improvement of cardiac functions.

Key words: Rostral ventrolateral medulla;Myocardial ischemia;Electroacupuncture;Neuronal nitric oxide synthase;Inducible nitric oxide synthase

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Citing This Article：

Xia Chunmei, Chen Jun, Wang Jin, Fan Mingxin, Xiao Fen, Cao Yinxiang, Li Li, Shen Linlin, Zhu Danian. Differential expressions of nNOS and iNOS in the rostral ventrolateral medulla induced by electroacupuncture in acute myocardial ischemia rats. Acta Physiol Sin 2008; 60 (4): 453-461 (in Chinese with English abstract).