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Proto--oncogene {sl c--src} regulates the viability of rat spermatogonial stem cells {sl in vitro} through phosphorylated signal transducer and activator of transcription--3

Chen Jiaxiang, Wang Xinchang, Wang Jinglei, Xu Sifan, Qin Haiyan, Yang Bei, Yang Junling, Zou Ting

Department of Physiology,Medical College of Nanchang University.Nanchang 330006,Jiangxi；China

Abstract

The present study aimed to investigate the effect of proto-oncogene c-src on the viability of rat spermatogonial stem cells in 9 day-old rat. MTT method was used to observe the viability of the spermatogonial stem cells treated with antisense src oligodeoxynucleotides (ODNs) in vitro; RT-PCR was utilized to observe the expressions of c-src mRNA and Western blot was used to observe the PP60c-src and phosphorylated signal transducer and activator of transcription-3 (p-STAT3) contents. Compared with that in control group, the viability of spermatogonial stem cells decreased by 8.1% (P<0.05) and the expression of c-src mRNA decreased significantly after being treated with 10 #mu#mol/L antisense src ODNs for 12 h. Compared with the control group, the PP60c-src and p-STAT3 contents decreased by 33.8% and 45.3% (both P<0.01), respectively, in the spermatogonial stem cells after transfected antisense c-src ODNs. The results suggest that proto-oncogene c-src regulates the viability of rat spermatogonia stem cells through p-STAT3.

Key words: Rat;spermatogonial stem cells;proto-oncogene c-src;signal transducer and activator of transcription-3

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Citing This Article：

Chen Jiaxiang, Wang Xinchang, Wang Jinglei, Xu Sifan, Qin Haiyan, Yang Bei, Yang Junling, Zou Ting. Proto--oncogene {sl c--src} regulates the viability of rat spermatogonial stem cells {sl in vitro} through phosphorylated signal transducer and activator of transcription--3. Acta Physiol Sin 2008; 60 (3): 391-396 (in Chinese with English abstract).