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IQ domain GTPase--activating protein 1 mediates the process of injury and repair in bronchial epithelial cells

Wang Yongping, Wang Fang, Wang Manxiang, Zhu Min, Ma Yan, Wu Renliang

Institute of Pathology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Key Laboratory of Pulmonary Disease of Ministry of Health of China.Wuhan 430030,Hubei

Abstract

The process of injury and repair in airway epithelium involves spreading and migration followed by cell proliferation. IQ domain GTPase-activating protein 1 (IQGAP1) acts in a series of cell processes, but has not been identified in lung epithelial cells. In this study, we utilized a widely used model in vitro by scratching bronchial epithelial cells (BECs) and investigated the function of IQGAP1. The results shower that IQGAP1 was abundant in BECs of several species. It was colocalized with tubulin cytoskeleton, but was destroyed by nocodazole, a microtubule disassembled reagent. IQGAP1 mRNA and protein expressions increased at 6-9 h after scratching. In addition, enforced overexpression of IQGAP1 translocated #beta#-catenin from the cytoplasm into the nucleus and activated the Tcf/Lef signal. Scratching altered the associations of IQGAP1 with #beta#-catenin, adenomatous polyposis coli (APC) and cytoplasmic linker protein-170 (CLIP-170). Depletion of IQGAP1 by small interference RNA (siRNA) blocked the wound closure. It is conclude that IQGAP1 signal is involved in the wound closure of BECs induced by scratching.

Key words: IQ domain GTPase-activating protein 1;injury and repair;bronchial epithelial cells

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Citing This Article：

Wang Yongping, Wang Fang, Wang Manxiang, Zhu Min, Ma Yan, Wu Renliang. IQ domain GTPase--activating protein 1 mediates the process of injury and repair in bronchial epithelial cells. Acta Physiol Sin 2008; 60 (3): 409-418 (in Chinese with English abstract).