Issue Archive

Analyses of exercise-induced muscle damage-specific microRNA expression and molecular target of sarcolemmal damage in rats

XU Yu-Ming, CAO Jian-Min, LI Jun-Ping, HUANG Qiao-Ting, WANG Ping

Sports and Health College of Hangzhou Normal University, Hangzhou 310036, China； Sport Science College of Beijing Sport University, Beijing 100086, China

Abstract

In the present study, we were to screen the specific microRNA (miRNA) of exercise-induced muscle damage (EIMD) and assess the EIMD-specific miRNAs-regulated target of sarcolemmal damage in rats. Twenty-four male Sprague-Dawley (SD) rats were randomly divided into 3 groups, which included sedentary (C), 24 h post-exercise (E24) and 48 h post-exercise (E48) groups. Rat EIMD model was established by an acute eccentric exercise, i.e., a downhill running treatment at −16&ordm; gradient. EIMD characteristics were verified by Evans blue dye staining, differentially expressed miRNAs were detected by microarray assay, EIMD-specific miRNAs expressions were further validated by real-time quantitative RT-PCR (RT-qPCR), and targets of the miRNAs were predicted based on mRNA expressions of associated proteins and related pathway core molecules of sarcolemmal damage. Two EIMD-specific expressed miRNAs, including miR-206-3p and miR-139-3p, were found in the study. There was a significantly negative correlation (P < 0.05) between miR-206-3p expression and dystrophin (r = −0.68), utrophin (r = −0.64), JNK (r = −0.62) or ERK1 (r = −0.68) respectively, but no correlation was found between miR-139-3p and these biomolecules. The results suggest that: i) the expression profile of miRNAs in rat is significantly affected by EIMD, ii) miR-206-3p and miR-139-3p are the EIMD-specific miRNAs, and iii) miR-206-3p may control sarcolemmal damage by regulating dystrophin, utrophin, JNK and ERK1.

Key words: Exercise; skeletal muscle ; damage ; sarcolemma ; microRNA

Received: 2016-12-01　　Accepted: 2016-12-27

Corresponding author: 徐玉明　　E-mail: xuyuming110@126.com

Citing This Article：

XU Yu-Ming, CAO Jian-Min, LI Jun-Ping, HUANG Qiao-Ting, WANG Ping. Analyses of exercise-induced muscle damage-specific microRNA expression and molecular target of sarcolemmal damage in rats. Acta Physiol Sin 2017; 69 (3): 276-284 (in Chinese with English abstract).