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Natriuretic peptide precursor C coding gene contributes to zebrafish angiogenesis

ZHANG Jing-Jing, WANG XIN, LIU Dong

Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China； Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, China

Abstract

This study aimed to investigate the expression of the natriuretic peptide precursor C coding gene nppc and its role in angiogenesis during embryonic period of the zebrafish. Whole mount in situ hybridization was performed to detect the expression pattern of nppc. nppc specific morpholino and nppc mRNA were injected respectively into the one-cell stage embryo to specifically knock-down and rescue the expression of nppc in Tg (flk1:GFP) and Tg (fli1a:nGFP) transgenic lines. The morphology and endothelial cell number of intersegmental vessel (ISV) were analyzed after imaging using the laser scanning confocal microscope. The results revealed that nppc was expressed in the brain, heart and vasculature of zebrafish larvae at 24 and 48 hours post-fertilization (hpf). Knock-down of nppc affected the development of ISV. Endothelial cell number was reduced after the knock-down of nppc. These results suggest that nppc controls zebrafish angiogenesis by affecting the endothelial cell proliferation and migration.

Key words: nppc; whole mount in situ hybridization ; angiogenesis ; zebrafish

Received: 2016-07-29　　Accepted: 2016-08-29

Corresponding author: 张晶晶,刘东　　E-mail: jingjing.zhang@live.com, liudongtom@gmail.com

Citing This Article：

ZHANG Jing-Jing, WANG XIN, LIU Dong. Natriuretic peptide precursor C coding gene contributes to zebrafish angiogenesis. Acta Physiol Sin 2017; 69 (1): 11-16 (in Chinese with English abstract).