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Lipid-lowering effect of seven traditional Chinese medicine monomers in zebrafish system

CHEN Kan, WANG Chang-Qian^*, FAN Yu-Qi, HAN Zhi-Hua, WANG Yue, GAO Lin, ZENG Hua-Su

Department of Cardiology, Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China

Abstract

The present study aimed to study lipid-lowering effect of seven traditional Chinese medicine monomers in zebrafish system. Zebrafish were fed with high fat diet to establish a hyperlipemia model, then fasted and bathed with seven traditional Chinese medicine monomers stigmasterol, triacontanol, chrysophanol, vanillic acid, shikimic acid, polydatin and oleanolic acid respectively. The oil red O staining was used to detect the blood lipids of zebrafish. Serum total cholesterol and triglyceride levels were detected to validate the lipid-lowering effect. The result showed that a zebrafish model of hyperlipemia could be established by feeding larvae zebrafish with high fat diet. Among the seven traditional Chinese medicine monomers, chrysophanol had lipid-lowering effect. Chrysophanol significantly reduced serum total cholesterol and triglyceride levels in adult zebrafish fed with high fat diet. Chrysophanol accelerated peristalsis frequency of zebrafish intestine and the excretion of high fat food. It is concluded that chrysophanol has lipid- lowering effect in zebrafish, and the mechanism of the effect may be due to the roles of chrysophanol in reducing lipid absorption from gastrointestinal tract and accelerating the excretion of food.

Key words: zebrafish; blood lipid ; traditional Chinese medicine monomer ; chrysophanol

Received: 2016-07-31　　Accepted: 2016-09-02

Corresponding author: 王长谦　　E-mail: wcqian@hotmail.com

Citing This Article：

CHEN Kan, WANG Chang-Qian, FAN Yu-Qi, HAN Zhi-Hua, WANG Yue, GAO Lin, ZENG Hua-Su. Lipid-lowering effect of seven traditional Chinese medicine monomers in zebrafish system. Acta Physiol Sin 2017; 69 (1): 55-60 (in Chinese with English abstract).