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[Activity of glial cells in the olfactory bulb of Niemann-Pick disease type C1 mice.] [Article in Chinese]

YAN Xin, QIAO Liang, Yang En-Hui, LIN Jun-Tang

College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, China； Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang 453003, China

Abstract

To study the pathological mechanisms of Niemann-Pick disease type C1, we observed the changes of activation of glial cells in the olfactory bulb of Npc1 mutant (Npc1−/−) mice. The genomic DNA was extracted from mouse tails for genotyping by PCR. Immunofluorescent histochemistry was performed to examine the activation of microglia and astrocytes in the olfactory bulb of Npc1−/− mice on postnatal day 30. NeuN, phosphorylated neurofilament (NF), Doublecortin (DCX), CD68 and GFAP were detected by Western blot. The results showed that Npc1 gene mutation strongly increased the activation of astrocytes and microglia in olfactory bulb associated with increased protein levels of CD68 and GFAP. Furthermore, the expression of phosphorylated NF was also significantly increased in the olfactory bulb of Npc1−/− mice compared with that in Npc1+/+ mice. However, DCX expression was significantly reduced. The above results suggest that there are some early changes in the olfactory bulb of Npc1−/− mice.

Key words: Niemann-Pick disease type C1; olfactory defects; microglia; astrocyte; glial cell

Received: 2015-10-19　　Accepted: 2016-01-12

Corresponding author: 林俊堂　　E-mail: linjtlin@126.com

Citing This Article：

YAN Xin, QIAO Liang, Yang En-Hui, LIN Jun-Tang. [Activity of glial cells in the olfactory bulb of Niemann-Pick disease type C1 mice.] [Article in Chinese]. Acta Physiol Sin 2016; 68 (2): 141-147 (in Chinese with English abstract).