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[Isoliquiritigenin relaxes the cerebral basilar artery by enhancing BKCa current in spontaneously hypertensive rat: role of sGC/cGMP .] [Article in Chinese]

TIAN Wei-Wei, Zhao Lei, MA Ke-Tao, LI Li, SI Jun-Qiang^*

Department of Physiology and Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University Medical College, Shihezi 832002, China

Abstract

The purpose of the present study is to investigate the effect of isoliquiritigenin (ISL) on the cerebral basilar artery in spontaneously hypertensive rats (SHR). The change of SHR systolic pressure was measured by tail artery pressure measurement instrument before and after ISL intervention. After perfusion with 1 × 10−5 mol/L phenylephrine (PE), 1 × 10−5 mol/L PE + 1 × 10−4 mol/L ISL and 1 × 10−5 mol/L PE, the diameter of the cerebral basilar artery separated from SHR was measured by pressure myograph. The current of large-conductance calcium-activated potassium (BKCa) channel of SHR single vascular smooth muscle cell (VSMC) was recorded by whole-cell patch-clamp technique and the cGMP levels of basilar artery was evaluated by ELISA. The results showed that 1) after intervention with ISL for 14 days, the systolic pressure of SHR was decreased from (218.3 ± 1.6) mmHg to (119.2 ± 1.9) mmHg (P < 0.01), but there was no difference in systolic pressure between ISL-treated SHR and Wistar-Kyoto (WKY) rat; 2) 1 × 10−4 mol/L ISL relaxed the SHR cerebral basilar artery (P < 0.01); 3) ISL significantly increased the outward current density of VSMC from SHR cerebral basilar artery (P < 0.01, n = 6), and the effect could be reversed by 1 × 10−3 mol/L TEA (a BKCa channel inhibitor), but 3 × 10−4 mol/L 4-AP (a Kv channel inhibitor) had no effect on the enhanced current density induced by ISL in VSMC; 4) 1 × 10−5 mol/L Methylene blue (a sGC inhibitor) significantly inhibited the ISL-enhanced current density in VSMC (P < 0.05, n = 6); 5) ISL significantly increased the cGMP level of SHR basilar artery (P < 0.05, n = 6). The results suggest that the role of the ISL in relaxing the SHR cerebral basilar artery may be related to its effect in enhancing BKCa current by increasing the levels of cGMP in the VSMC.

Key words: isoliquiritigenin; basilar artery; BKCa channel; cGMP

Received: 2014-12-09　　Accepted: 2015-01-23

Corresponding author: 司军强　　E-mail: sijunqiang@shzu.edu.cn

Citing This Article：

TIAN Wei-Wei, Zhao Lei, MA Ke-Tao, LI Li, SI Jun-Qiang. [Isoliquiritigenin relaxes the cerebral basilar artery by enhancing BKCa current in spontaneously hypertensive rat: role of sGC/cGMP .] [Article in Chinese]. Acta Physiol Sin 2015; 67 (3): 329-334 (in Chinese with English abstract).