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Intraventricular injection of 5,7-dihydroxytryptamine alters neuronal activity of neurons in the medial prefrontal cortex of rat

FAN Ling-Ling^*, HU Zhi-Hong, ZHOU Yu-Dong, LI Qian-Qian, JIA Yan-Ru, WANG Jin-Feng, ZHAO Chun-Wei

Medical College, Henan University of Science and Technology, Luoyang 471003, China

Abstract

The present study is aimed to investigated the firing activity of pyramidal neurons and interneurons in the medial prefrontal cortex (mPFC) in rats with bilateral intraventricular injection of 5,7-dihydroxytryptamine (5,7-DHT) by using in vivo extracellular recording. The results showed that the injection of 5,7-DHT reduced the 5-hydroxytryptamine (5-HT) levels in the mPFC and dorsal raphe nucleus in the rats. The firing rate of mPFC pyramidal neurons in rats with 5,7-DHT injection was significantly higher than that of normal rats, and the firing pattern of these neurons also changed significantly towards a more burst-firing, while the injection decreased the firing rate of mPFC interneurons and changed the firing pattern of the interneurons towards a more irregular. These results indicate that the lesions of the serotonergic neurons lead to the changes in the firing activity of mPFC pyramidal neurons and interneurons, suggesting that serotonergic system plays an important role in the regulation of the neuronal activity in the mPFC.

Key words: medial prefrontal cortex; 5,7-hihydroxytryptamine; pyramidal neuron; interneuron; electrophysiology; rat

Received: 2014-05-09　　Accepted: 2014-07-15

Corresponding author: 范玲玲　　E-mail: emmafll@163.com

Citing This Article：

FAN Ling-Ling, HU Zhi-Hong, ZHOU Yu-Dong, LI Qian-Qian, JIA Yan-Ru, WANG Jin-Feng, ZHAO Chun-Wei. Intraventricular injection of 5,7-dihydroxytryptamine alters neuronal activity of neurons in the medial prefrontal cortex of rat. Acta Physiol Sin 2014; 66 (6): 667-674 (in Chinese with English abstract).