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[Effect of kinetin on immunity and splenic lymphocyte proliferation in vitro in D-galactose-induced aging rats.] [Article in Chinese]

LI Meng-Yun, OUYANG Wu-Qin, WU Xiao-Li, ZHENG Yi, GAO Rui, TANG Jia-Xin

College of Veterinary Medicine, Northwest A&；F University, Yangling 712100, China； College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China

Abstract

The purpose of this paper is to study the effect of kinetin (Kn) on immunity and splenic lymphocyte proliferation in vitro of aging rats induced by D-galactose (D-gal). Fifty SD rats were randomly divided into five groups: control group, aging model group, Kn low dose group, Kn middle dose group and Kn high dose group. The aging model group was proposed by napes subcutaneous injection of D-gal (125 mg/kg) for 45 d, and anti-aging groups were intragastrically administered with 5, 10, 20 mg/kg of Kn respectively from day 11. IgG, IgA, IgM contents of serum, the apoptosis percentage, stimulation index (SI) and proliferation index (PI) of splenic lymphocyte in vitro were evaluated. The results showed that the apoptosis percentage of splenic lymphocyte in aging model rats was higher, the serum IgG, IgA and IgM contents, SI and PI were lower than control group. Kn significantly decreased the apoptosis percentage of splenic lymphocyte, while increased the serum IgG, IgA and IgM contents, SI and PI in aging model group. These results suggest that Kn could inhibit the apoptosis, while promote the proliferation of splenic lymphocyte, and then effectively enhance the immune power of the aging rats and slow down the aging process.

Key words: kinetin; spleen; anti-aging; immunity; proliferation

Received: 2014-01-18　　Accepted: 2014-04-17

Corresponding author: 欧阳五庆　　E-mail: oywq506@sina.com

Citing This Article：

LI Meng-Yun, OUYANG Wu-Qin, WU Xiao-Li, ZHENG Yi, GAO Rui, TANG Jia-Xin. [Effect of kinetin on immunity and splenic lymphocyte proliferation in vitro in D-galactose-induced aging rats.] [Article in Chinese]. Acta Physiol Sin 2014; 66 (5): 605-611 (in Chinese with English abstract).