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[Estrogen reduced myocardial damage by regulating Gαs-cAMP pathway in isoprenaline injured rats.] [Article in Chinese]

SANG Li-Li, ZHOU Chun-Le, FU Lu, CHONG Jing-Min, ZHANG Lin, CAO Xian-Cun, SUN Hong^*

Department of Physiology, Xuzhou Medical College, Xuzhou 221004, China

Abstract

The aim of the present study is to explore the mechanism of estrogen on regulating cardiac function disorder by adjusting the stimulating adenylate cyclase G α protein (Gαs)-cycle adenosine monophosphate (cAMP) signal pathway. Adult female rats were randomly divided into five groups: sham group, ovariectomized group (OVX), OVX and 17β-estradiol given group (OVX+E2), OVX and isoprenaline injected group (OVX+ISO), OVX and 17β-estradiol, isoprenaline injected group (OVX+E2+ISO). Rats were ovariectomized, and two weeks later, OVX+E2 group was injected with E2, OVX+ISO group was injected with ISO, OVX+E2+ISO group was injected with E2 and ISO. Another four weeks later, the hemodynamic parameters were monitored by carotid artery intubation: left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), maximal differentials of left ventricular developed pressure (+dp/dtmax), and minimal differentials of left ventricular developed pressure (−dp/dtmax). Brain natriuretic peptide (BNP) and cAMP concentration in plasma were determined; Gαs protein expression in myocardium was determined. The results showed that the hemodynamic parameters, the concentration of BNP and cAMP in plasma had no significant changes after ovariectomy compared with sham group. But after isoprenaline injection in ovariectomized rats, LVSP and +dp/dtmax declined (P < 0.01), LVEDP and −dp/dtmax elevated (P < 0.01); plasma BNP concentration increased (P < 0.01); plasma cAMP concentration decreased (P < 0.01), compared with OVX group. Further estrogen supplements improved the heart function treated by isoprenaline: LVSP and +dp/dtmax elevated (P < 0.01), LVEDP and −dp/dtmax declined (P < 0.05, P < 0.01); the plasma BNP concentration decreased (P < 0.01); the plasma cAMP concentration increased (P < 0.01). Estrogen had no significant influence on Gαs protein expression. The results suggest that estrogen can alleviate myocardial injury and regulate cardiac function disorder by increasing cAMP level, finally improved the excessive suppression of myocardium.

Key words: Estrogen; isoprenaline; Gαs protein; cAMP

Received: 2014-02-28　　Accepted: 2014-04-30

Corresponding author: 孙红　　E-mail: sunh@xzmc.edu.cn

Citing This Article：

SANG Li-Li, ZHOU Chun-Le, FU Lu, CHONG Jing-Min, ZHANG Lin, CAO Xian-Cun, SUN Hong. [Estrogen reduced myocardial damage by regulating Gαs-cAMP pathway in isoprenaline injured rats.] [Article in Chinese]. Acta Physiol Sin 2014; 66 (5): 583-588 (in Chinese with English abstract).