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[Upregulation of P2X3 receptor in dorsal root ganglion of TRPV1 knockout female mice.] [Article in Chinese]

FANG Xiao, SHI Xiao-Han, HUNG Li-Bin, RONG Wei-Fang, MA Bei

Department of Physiology, Secondary Military Medical University, Shanghai 200433, China； Department of Physiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200003, China

Abstract

The study was aimed to investigate the changes in mechanical pain threshold in the condition of chronic inflammatory pain after transient receptor potential vanilloid 1 (TRPV1) gene was knockout. Hind-paw intraplantar injection of complete freund's adjuvant (CFA, 20 μL) produced peripheral inflammation in wild-type and TRPV1 knockout female mice. The mechanical pain thresholds were measured during the 8 days after injection and pre-injection by using Von-Frey hair. Nine days after injection, mice were killed and the differences of expression of c-Fos and P2X3 receptor in the dorsal root ganglia (DRG) and spinal cord dorsal horn were examined by Western blotting between the two groups. Compared with that in wild-type mice, the mechanical pain threshold was increased significantly in TRPV1 knockout mice (P < 0.05); 3 days after CFA injection, the baseline mechanical pain threshold in the TRPV1 knockout mice group was significantly higher than that in the wild-type mice group (P < 0.05); The result of Western blotting showed that the expression of c-Fos protein both in DRG and spinal cord dorsal horn of TRPV1 knockout mice group was decreased significantly compared with that in wild-type mice group (P < 0.01, P < 0.05), while the expression of P2X3 receptor in DRG of TRPV1 knockout mice group was increased significantly compared with that in wild-type mice group (P < 0.05). Our findings indicate that TRPV1 may influence the peripheral mechanical pain threshold by mediating the expression of c-Fos protein both in DRG and spinal cord dorsal horn and changing the expression of P2X3 receptor in DRG.

Key words: TRPV1 cation channels; P2X3 receptor; knockout mice; spinal ganglia; c-Fos protein; pain threshold

Received: 2013-11-30　　Accepted: 2014-05-04

Corresponding author: 马蓓　　E-mail: mabei08@aliyun.com

Citing This Article：

FANG Xiao, SHI Xiao-Han, HUNG Li-Bin, RONG Wei-Fang, MA Bei. [Upregulation of P2X3 receptor in dorsal root ganglion of TRPV1 knockout female mice.] [Article in Chinese]. Acta Physiol Sin 2014; 66 (4): 431-437 (in Chinese with English abstract).