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[Neuropathic pain enhances expression of HCN2 channel in rat CSF-contacting nucleus.] [Article in Chinese]

WU Tong, CAO Jing, ZHANG Li-Cai^*

Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, Xuzhou 221004, China

Abstract

The purpose of this research is to explore the distribution and expression of hyperpolarization-activated cyclic nucleotide-gated channels subtype 2 (HCN2) in cerebrospinal fluid (CSF)-contacting nucleus in neuropathic pain, and provide experimental evidence to reveal the biological function and regulation mechanisms of CSF-contacting nucleus in neuropathic pain. Neuropathic pain model was produced by chronic constriction injury (CCI) in Sprague-Dawley (SD) rats. Intracerebroventricular injection of cholera toxin subunit B (CTb) labeled with horseradish peroxidase (CB-HRP) was used to specifically mark distal CSF-contacting nucleus. The thermal withdrawal latency and mechanical withdrawal threshold of rats were recorded to detect the change of pain threshold. The expressions HCN2 channel and c-Fos proteins in CSF-contacting nucleus were detected by immunofluorescence and Western blot. The results showed that, compared with the control group, CTb-treated rats did not show any differences in the expressions of HCN2 channel and c-Fos proteins in CSF-contacting nucleus, as well as pain threshold. At 7, 14 d after CCI operation, the model rats showed not only significantly increased expressions of HCN2 channel and c-Fos in CSF-contacting nucleus, but also decreased pain threshold. ZD7288, a HCN2 channel blocker, could reverse the above changes in neuropathic pain model rats. These results suggest that the CSF-contacting nucleus may be involved in the process of neuropathic pain via the HCN2 channel.

Key words: CSF-contacting nucleus; hyperpolarization-activated cyclic nucleotide-gated channels subtype 2; ion channel; c-Fos; ZD7288; neuropathic pain

Received: 2013-12-17　　Accepted: 2014-02-10

Corresponding author: 张励才　　E-mail: lczhang@xzmc.edu.cn

Citing This Article：

WU Tong, CAO Jing, ZHANG Li-Cai. [Neuropathic pain enhances expression of HCN2 channel in rat CSF-contacting nucleus.] [Article in Chinese]. Acta Physiol Sin 2014; 66 (3): 323-331 (in Chinese with English abstract).