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[MCPIP1 mediates MCP-1-induced vascular smooth muscle cell proliferation.] [Article in Chinese]

YANG Yi-Jun, WU Liu-Song, SHU Bo, QIAN Min-Zhang^*

Department of Biochemistry, Zunyi Medical College, Zunyi 563003, China

Abstract

The aim of the present study is to investigate whether monocyte chemotactic protein-1 (MCP-1)-induced vascular smooth muscle cell (VSMC) proliferation is mediated via monocyte chemotactic protein-1 induced protein-1 (MCPIP1). MCPIP1 expressions in cultured VSMC were detected by real-time PCR and Western blot following MCP-1 incubation. After MCPIP1 was silenced by siRNA, cell number was counted by hemocytometer, VSMC activity was analyzed by CCK-8 kit, percentage of DNA synthesis was detected by EdU kit, percentage of S phase cell numbers were measured by flow cytometry, and c-fos mRNA expression induced by MCP-1 or platelet-derived growth factor (PDGF) was detected by real-time PCR. The results showed MCP-1 increased MCPIP1 mRNA and up-regulated MCPIP1 protein expression in dose- and time-dependent manners. Cell counts, cellular activity, the percentage of DNA synthesis, and the percentage of S phase cell numbers were remarkably decreased in MCPIP1 siRNA group, compared with those in MCP-1 group. The enhancing effect of MCP-1 or PDGF on c-fos mRNA expression was inhibited by MCPIP1 siRNA. These results suggest that MCP-1-induced VSMC proliferation is mediated via MCPIP1, and the underlying mechanism may involve c-fos expression up-regulation.

Key words: monocyte chemotactic protein-1; monocyte chemotactic protein-1 induced protein-1; vascular smooth muscle cell proliferation; c-fos

Received: 　　Accepted:

Corresponding author: 钱民章　　E-mail: qian_mzh@hotmail.com

Citing This Article：

YANG Yi-Jun, WU Liu-Song, SHU Bo, QIAN Min-Zhang. [MCPIP1 mediates MCP-1-induced vascular smooth muscle cell proliferation.] [Article in Chinese]. Acta Physiol Sin 2013; 65 (6): 616-622 (in Chinese with English abstract).