Issue Archive

[Involvement of store-operated calcium channels and receptor-operated calcium channels in Ca2+-sensing receptor-evoked extracellular Ca2+ influx and NO generation in human umbilical vein endothelial cells.] [Article in Chinese]

ZHAO Hui, LIANG Xiao, ZHONG Hua, ZHANG Chun-Jun, HE Fang

Department of Pathophysiology/Key Laboratory of Education Ministry of Xinjiang Endemic and Ethnic Diseases, Medical College of Shihezi University, Shihezi 832002, China； Department of lymphoma and breast medicine, Affiliated Tumor Hospital, Xinjiang Medcial University, Urumqi 830011, China

Abstract

This paper aims to investigate the effect of store-operated calcium channels (SOC) and receptor-operated calcium channels (ROC) on Ca²⁺-sensing receptor (CaR)-induced extracellular Ca²⁺ influx and nitric oxide (NO) generation in human umbilical vein endothelial cells (HUVEC). SOC blocker, non-selective cation channel blocker, ROC agonist and ROC blocker were used separately and combined. Intracellular Ca²⁺ concentration ([Ca²⁺]_i) was measured by Fura-2/AM loading. The activity of endothelial nitric oxide synthase (eNOS) and the production of NO were determined by the DAF-FM diacetate (DAF-FM DA). The results showed that increases of [Ca²⁺]_i, eNOS activity and NO generation induced by CaR agonist Spermine were all reduced after single blocking the SOC or ROC, respectively (P < 0.05). ROC agonist can partially abolish the ROC blocker's effect (P < 0.05). The above mentioned effects evoked by CaR agonist Spermine were further reduced when blocking both SOC and ROC than single blocking SOC or ROC in HUVEC (P < 0.05). In conclusion, these results suggest that the SOC and ROC participate in the processes of CaR-evoked extracellular Ca²⁺ influx and NO generation by a synergistic manner in HUVEC.

Key words: Endothelial cells; calcium-sensing receptor; calcium signaling; nitric oxide; store-operated calcium channels; receptor-operated calcium channels

Received: 2013-02-27　　Accepted: 2013-08-16

Corresponding author: 　　E-mail:

Citing This Article：

ZHAO Hui, LIANG Xiao, ZHONG Hua, ZHANG Chun-Jun, HE Fang. [Involvement of store-operated calcium channels and receptor-operated calcium channels in Ca2+-sensing receptor-evoked extracellular Ca2+ influx and NO generation in human umbilical vein endothelial cells.] [Article in Chinese]. Acta Physiol Sin 2013; 65 (5): 553-561 (in Chinese with English abstract).