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Effect of CCK receptor antagonists on plasma CCK bioassay

SUN Gang, PAN Guo-Zong, MA Fang, WEN Shu-Hao, DU Shou-Fen

Beijing Union Medical College Hospital, Beijing 100730, China

Abstract

利用生物法测定了102例正常人空腹及进食二个油煎鸡蛋30min后血浆CCK浓度,结果分别为1.4±0.1pmol/L和4.9±0.4pmol/L（X±SE）。血浆CCK浓度不随性别和年龄而变化。本文研究了三种不同类型的CCK受体拮抗剂对CCK生物测定的影响。一组将L364,718（5.0nmol/L）,丙谷胺（1.0mmol/L）或Bt₂-cGMP（0.1mmol/L）分别加入含8pmol/LCCK-8的人血浆,用SEP-PAK提取,另一组先提取血浆,于测定前向血浆提取物内加入上述拮抗剂,两组同时测定。结果显示,在有L364,718存在时仍可利用生物法测定血浆CCK浓度,如血中含有丙谷胺或Bt₂-cGMP则无法准确测定。

Key words: cholecystokinin (CCK); bioassay; CCK antagonist

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Citing This Article：

SUN Gang, PAN Guo-Zong, MA Fang, WEN Shu-Hao, DU Shou-Fen. Effect of CCK receptor antagonists on plasma CCK bioassay. Acta Physiol Sin 1992; 44 (6): 609-613 (in Chinese with English abstract).