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[Role of UGBP in Antiflammin-1 promoting IL-10 expression and secretion in RAW264.7 cells induced by endotoxin.] [Article in Chinese]

ZHANG Tian-Jie, HAN Jian-Zhong, LIU Hiu-Jun, LIAO xiao-Hong, LI Chen, LUO Zi-Qiang

1Department of Physiology, Central South University, Changsha 410078, China； 2Department of Physiology, Xiangnan College, Chenzhou 423000, China； 3Department of Physiology, Changzhi Medical College, Changzhi 046000, China

Abstract

The present study investigated the effect of antiflammin-1 (AF-1) on LPS-induced IL-10 secretion from RAW264.7 cells through uteroglobin-binding protein (UGBP). Cultured RAW264.7 cells, a murine monocyte-macrophage cell line, were divided as following: control group, LPS group (1 µg/mL LPS), AF-1 group (100 μmol/L AF-1), LPS+AF-1 group (2 h of 100 μmol/L AF-1 pretreatment before LPS addition), and LPS+AF-1+anti-UGBP group (30 min of anti-UGBP antibody pretreatment before successive treatments with AF-1 and LPS). IL-10 concentration in the supernatants was detected by ELISA assay, and the level of IL-10 mRNA expression in macrophage was detected by using RT-PCR method. The results showed that AF-1 significantly increased LPS-induced IL-10 secretion in RAW264.7 cells in a dose dependent way, and up-regulated its mRNA level. Anti-UGBP antibody pretreatment attenuated the augmented effect of AF-1 on LPS-induced IL-10 secretion and gene expression. These results suggest that AF-1 promotes LPS-induced IL-10 secretion from macrophages, and this effect is mediated by UGBP.

Key words: antiflammin-1; uteroglobin; lipopolysaccharide; interleukin-10

Received: 2013-01-17　　Accepted: 2013-07-02

Corresponding author: 李晨,罗自强　　E-mail: luozq1962@163.com, ivyandl.li@hotmail.com

Citing This Article：

ZHANG Tian-Jie, HAN Jian-Zhong, LIU Hiu-Jun, LIAO xiao-Hong, LI Chen, LUO Zi-Qiang. [Role of UGBP in Antiflammin-1 promoting IL-10 expression and secretion in RAW264.7 cells induced by endotoxin.] [Article in Chinese]. Acta Physiol Sin 2013; 65 (4): 363-369 (in Chinese with English abstract).