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[Diverse expression of ER-α36, a novel variant of ER-α, in hippocampus and cortex of neonatal and adult rats.] [Article in Chinese]

Liu Yang, FANG Chen, ZOU Ping, MA Yi-Ni, HAN Dan-Nv, JI Zhi-Hong, LIANG Xiao-Feng, GUAN Xin, HUANG Liang, FENG Tian, WANG Yi-Ting, LIU Jing, ZOU Wei

College of Life Science, Liaoning Normal University, Dalian 116081, China； The Research Center of Developmental and Educational Psychology, Liaoning Normal University, Dalian 116029, China； Regenerative Medicine Centre, First Affiliated Hospital of Dalian Medical University, Dalian 116011, China； Teacher's College, Dalian University, Dalian 116622, China； Medical College, Dalian University, Dalian 116622, China； School of Life Science, Fudan University, Shanghai 200433, China. E-mail: weizou60@126.com； liujing.dlrmc@hotmail.com

Abstract

ER-α36, a novel variant of ER-α, is expressed in breast, uterus, digestive tract, respiratory tract etc. The aim of the present study was to investigate the distribution and expression of ER-α36 in the central nervous system (CNS). Here, we comparatively analyzed the expression pattern of ER-α36 in the hippocampus and cortex of neonatal (1-day-old) and adult (12-week-old) Sprague-Dawley (SD) rats by using immunohistochemistry/immunocytochemistry analysis and Western blot. The results showed that ER-α36 was expressed both in hippocampus and cortex of adult rats, but mainly distributed in pyramidal neurons. ER-α36 was mainly located on the cytomembrane of hippocampal and cortical neurons from neonatal rats. Compared with the cortical neurons, the hippocampal neurons showed lower ER-α36 protein expression in the neonatal rats, but exhibited higher level of ER-α36 in the adult rats. Furthermore, the adult rats showed higher levels of ER-α36 expression in both hippocampus and cortex compared with the neonatal rats. These results suggest that ER-α36 might be involved in the regulation of membrane-initiated estrogen signaling throughout the postnatal development of diverse brain regions, and thus will be a potential target for the treatment of degenerative diseases in nervous system.

Key words: ER-α36; central nervous system; growth and development; primary culture

Received: 2012-09-04　　Accepted: 2013-01-06

Corresponding author: 邹伟　　E-mail: weizou60@126.com

Citing This Article：

Liu Yang, FANG Chen, ZOU Ping, MA Yi-Ni, HAN Dan-Nv, JI Zhi-Hong, LIANG Xiao-Feng, GUAN Xin, HUANG Liang, FENG Tian, WANG Yi-Ting, LIU Jing, ZOU Wei. [Diverse expression of ER-α36, a novel variant of ER-α, in hippocampus and cortex of neonatal and adult rats.] [Article in Chinese]. Acta Physiol Sin 2013; 65 (3): 263-268 (in Chinese with English abstract).