Changes of NMDA receptor expression in rat hippocampal formation after establishment of spatial memory by different training modes
ZHANG Li, YU Wei, HAN Tai-Zhen, XIE Wen, LUO Yu
Department of Physiology and Pathophysiology, Medical School of Xi’an Jiaotong University； Ministry of Education Key Laboratory of Environment and Gene Related Diseases, Xi’an Jiaotong University, Xi’an 710061, China； Department of Physiology, Xi’an Medical School, Xi’an 710068, China
Abstract
There is no unambiguous report so far on whether short reinforcing training could establish stable spatial long-term memoryand how the NMDA/NR1 expression in the hippocampal formation changes after establishment of short- and long-term memory. In thepresent study, three spatial memory training modes of Morris water maze were used: spatial long-term memory training mode (LTgroup), spatial short-term memory training mode (ST group) and short reinforcing training mode (SRT group). The characteristics ofthose memories established by different training modes were compared. The immunofluorescence histochemical staining technique wasused to observe the changes of NMDA/NR1 expression in the hippocampal formation in the three groups. The results showed that,during Morris water maze training, no significant difference was found in the mean latency and the strategies to the target between theLT and SRT groups. The examination of the memories following different trainings indicated that the mean latency and the strategies tothe target as well as the times crossing the target in LT group were not significantly different from those in SRT group, except that themean time remaining in the platform quadrant in LT group was significantly longer than that in SRT group. In addition, no significantdifference in the intensity of NMDA/NR1 immunoreactivity in CA1, CA3 and dentate gyrus of the hippocampal formation was observed between the control and ST groups or between LT and SRT groups. However, the intensity of NMDA/NR1 immunoreactivityin CA1 area and dentate gyrus in both LT and SRT groups was significantly increased as compared with that in ST or the controlgroups. These results suggest that the short reinforcing training mode can establish long-term spatial memory, which is almost the sameas that established by the long-term training mode, and that the increase of NMDA/NR1 expression in CA1 area and dentate gyrus ofthe hippocampal formation may be one of the mechanisms of spatial long-term memory formation.
Key words: Spatial learning and memory; water maze; immunofluorescence; hippocampal formation; NMDA receptor
Received: Accepted:
Corresponding author: 韩太真 E-mail: htzhen@mail.xjtu.edu.cn
Citing This Article：
ZHANG Li, YU Wei, HAN Tai-Zhen, XIE Wen, LUO Yu. Changes of NMDA receptor expression in rat hippocampal formation after establishment of spatial memory by different training modes. Acta Physiol Sin 2006; 58 (5): 442-448 (in Chinese with English abstract).
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