Issue Archive

[Protective effects of crude polysaccharides from Chroogomphus rutilus on SH-SY5Y cells impaired by MPP(+).] [Article in Chinese]

ZHANG Xue-Qian, WANG Yan-qin, WANG Li-An, GUAN Zhen-Long

College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China； Affiliated High School of Hebei Normal University, Shijiazhuang 050035, China

Abstract

The aim of the present study was to investigate the protective effects of crude polysaccharides from Chroogomphus rutilus on dopaminergic neurons impaired by MPP(+). SH-SY5Y cells were pretreated with crude polysaccharides (200, 400 and 800 μg/mL), and then MPP(+) was added to cell medium. After 48 h of incubation, MTT method was used to detect the survival rate of SH-SY5Y cells damaged by MPP(+). Annexin V-FITC staining and flow cytometry were used to detect apoptotic rate. The results showed that pretreating SH-SY5Y cells with crude polysaccharides (400 and 800 μg/mL) increased the survival rates, and reduced the apoptotic rates of SH-SY5Y cells. To rule out the possibility that crude polysaccharides may decrease actual concentration of MPP(+) by direct binding, we washed off crude polysaccharides before MPP(+) addition. Under this experimental condition, MTT results showed the survival rates of the SH-SY5Y cells were still significantly increased by 800 μg/mL crude polysaccharides pretreatment. These results suggest a protective effect of polysaccharides on the SH-SY5Y cells. Most of this protection is contributed by direct action of polysaccharides on the cells, not by binding with MPP(+). It is indicated that the crude polysaccharides from Chroogomphus rutilus can be developed as a potential drug for Parkinson's disease prevention and treatment in the future.

Key words: Parkinson’s disease; dopaminergic neuron; apoptosis; protective effects; antioxidant

Received: 2012-06-19　　Accepted: 2012-08-15

Corresponding author: 管振龙　　E-mail: zlguan@yahoo.com

Citing This Article：

ZHANG Xue-Qian, WANG Yan-qin, WANG Li-An, GUAN Zhen-Long. [Protective effects of crude polysaccharides from Chroogomphus rutilus on SH-SY5Y cells impaired by MPP(+).] [Article in Chinese]. Acta Physiol Sin 2013; 65 (2): 210-216 (in Chinese with English abstract).