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[Insulin promotes proliferation of skeletal myoblast cells through PI3K/Akt and MEK/ERK pathways in rats ] [Article in Chinese]

YU Huan, ZHANG Min, ZHAO Yong, WU Ping, CHEN Pei-liang, LI Wei-dong^*

The Systems Bio-medicine Key Laboratory of Jiangxi, Jiujiang University, Jiujiang 332000, China

Abstract

The present study was to explore the effects of insulin on proliferation of skeletal myoblast cells in rats. Separated and cultured primary skeletal myoblast cells from rats were treated by insulin. By means of the incorporation of (3)H-TdR, BrdU assay and MTT assay, the proliferation of skeletal myoblast cells was detected. Western blot was used to check the phosphorylation of Akt and ERK of myoblast cells. The results showed that insulin significantly promoted the incorporation of (3)H-TdR into cultured skeletal myoblast cells in a dose-dependent manner. MTT assay and BrdU assay also showed insulin promoted the proliferation of skeletal myoblast cells. The promotion of skeletal myoblast cells proliferation by insulin was inhibited by PI3K inhibitor wortmannin or MEK inhibitor U0126, and the same phenomenon was shown in L6 and C2C12 cells. Also, insulin increased the phosphorylation of Akt and ERK in myoblast cells. These results suggest that insulin may promote proliferation of skeletal myoblast cells through PI3K/Akt and MEK/ERK pathways.

Key words: skeletal myoblast cells;Insulin;Proliferation;PI3K/Akt;MEK/ERK

Received: 2012-05-20　　Accepted: 2012-08-29

Corresponding author: 李卫东　　E-mail: lwd62518@yahoo.com.cn

Citing This Article：

YU Huan, ZHANG Min, ZHAO Yong, WU Ping, CHEN Pei-liang, LI Wei-dong. [Insulin promotes proliferation of skeletal myoblast cells through PI3K/Akt and MEK/ERK pathways in rats ] [Article in Chinese]. Acta Physiol Sin 2013; 65 (1): 19-25 (in Chinese with English abstract).