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[Ameliorative effects of exogenous sulfur dioxide on lipopolysaccharide-induced acute lung injury in rats.] [Ariticle in Chinese]

HUANG Xin-Li, ZHOU Jun-Lin, ZHOU Xiao-Hong, XIAN Xiao-Hui, DING Chun-Hua

Department of Pathophysiology, Hebei Medical University, Shijiazhuang 050017, China； Department of Orthopaedics, Beijing Chaoyang Hospital Affiliated to Capital Medical University, Beijing 100020, China

Abstract

To investigate the influence of sulfur dioxide (SO2) on lipopolysaccharide (LPS)-induced acute lung injury (ALI), we examined the influence of exogenous SO2 on pulmonary tissue inflammatory response. A rat model of ALI induced by intravenous (IV) injection of LPS was developed. Male Sprague-Dawley (SD) rats were divided into four groups randomly: control group, LPS group, LPS plus SO2 group (IV injection of 0.5 mL Na2SO3/NaHSO3 10 min before LPS administration) and SO2 group (only given Na2SO3/NaHSO3). Animals were sacrificed 6 h after agent administration. Lung weight/body weight ratio (LW/BW) was measured and calculated. Morphological changes of lung tissues were observed. The number of polymorphonuclear neutrophil (PMN) in the bronchoalveolar lavage fluid (BALF), intercellular adhesion factor-1 (ICAM-1) expression in the lung tissue and IL-1, IL-6 and IL-10 levels in the serum were tested. The results showed that, compared to control rats, the LPS-treated rats had severe injuries of lung tissues and an increased LW/BW, increased index of quantitative assessment (IQA) score, increased PMN number in the BALF, increased ICAM-1 expression in the lung tissue and increased IL-1, IL-6 and IL-10 levels in the serum 6 h after LPS injection. Administration of the SO2 donor, Na2SO3/NaHSO3, into LPS-treated rats reduced the LW/BW, PMN number and ICAM-1 expression, and alleviated the degree of ALI (measured by the IQA score). In addition, Na2SO3/NaHSO3 decreased IL-1 and IL-6 levels, but increased IL-10 level in the serum. There were no significant differences in the above indexes between SO2-treated rats and control rats. These results suggest that exogenous SO2 could inhibit the pulmonary tissue inflammatory response in rats with LPS-induced ALI.

Key words: Endotoxin; lung injury; sulfur dioxide; rat

Received: 2009-07-08　　Accepted: 2009-09-07

Corresponding author: 周晓红　　E-mail: zxh19703@163.com

Citing This Article：

HUANG Xin-Li, ZHOU Jun-Lin, ZHOU Xiao-Hong, XIAN Xiao-Hui, DING Chun-Hua . [Ameliorative effects of exogenous sulfur dioxide on lipopolysaccharide-induced acute lung injury in rats.] [Ariticle in Chinese] . Acta Physiol Sin 2009; 61 (5): 499-503 (in Chinese with English abstract).