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[Ca2+-dependent potassium channels play important roles in regulatory volume decrease in human nasopharyngeal carcinoma cells.] [Ariticle in Chinese]

HE Shu-Tong, ZHU Lin-Yan, YANG Lin-Jie, HE Si-Chun, MAO Jian-Wen, WANG Li-Wei, CHEN Li-Xin

Department of Physiology； Department of Pharmacology, Medical College of Jinan University , Guangzhou 510632, China； Department of Biology, Guangdong Pharmaceutical University, Guangzhou 510006, China

Abstract

It has been shown that cell volume regulation mechanisms play important roles in various cell functions. We demonstrated previously that volume-activated chloride channels were involved in cell volume regulation. The present study aimed to clarify the roles of various types of potassium channels in regulatory volume decrease (RVD) induced by hypotonic challenges in human nasopharyngeal carcinoma cells (CNE-2Z cells). The whole-cell patch clamp technique was used to record hypotonic challenge-induced potassium currents. During current recordings, cells were held at 0 mV and stepped to ±46 and ±92 mV, repeatedly. The cell volume was computed from cell diameters. The changes of cell volume were monitored and analyzed by the time-lapse imaging technique. The results showed that the exposure to 160 mOsm/L hypotonic solution caused the cells to swell by (144.5±4.2)%, activated a potassium current (59.2 pA/pF±13.3 pA/pF at 92 mV), and induced RVD. Cell volume was recovered from hypotonic challenge-induced swelling by (48.9±4.6)% after 20 min. The potassium current (at 92 mV) and RVD were inhibited by the calcium-dependent potassium channel blocker, clotrimazole (100 μmol/L), by (98.5±2.8)% and (89.3±4.9)%, respectively. Depletion of extracellular calcium prevented the activation of the hypotonic challenge-induced potassium current and inhibited the process of RVD. The voltage-gated potassium channel blocker, 4-AP (5 mmol/L), partially inhibited the hypotonic challenge-activated potassium currents by (66.6±5.3)% (at 92 mV). These results suggest that the Ca2+-dependent potassium channel is the main component of volume-activated potassium channels and plays an important role in volume regulation of CNE-2Z cells. The voltage-gated potassium channels may also contribute in part to the formation of the volume-activated potassium current.

Key words: nasopharyngeal neoplasms; hypotonic solutions; cell size; potassium channels

Received: 2009-06-29　　Accepted: 2009-08-04

Corresponding author: 王立伟,陈丽新　　E-mail: wangliweic@sohu.com,

Citing This Article：

HE Shu-Tong, ZHU Lin-Yan, YANG Lin-Jie, HE Si-Chun, MAO Jian-Wen, WANG Li-Wei, CHEN Li-Xin. [Ca2+-dependent potassium channels play important roles in regulatory volume decrease in human nasopharyngeal carcinoma cells.] [Ariticle in Chinese] . Acta Physiol Sin 2009; 61 (5): 485-492 (in Chinese with English abstract).