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[Effects of parabolic flight on redox status in SH-SY5Y cells.] [Ariticle in Chinese]

BI Lei, QU Li-Na^*, HUANG Zeng-Ming, WANG Chun-Yan, LI Qi, TAN Ying-Jun, LI Ying-Hui

Laboratory of Space Cellular and Molecular Biology, China Astronaut Research and Training Center, Beijing 100094, China

Abstract

Space flight is known to produce a number of neurological disturbances. The etiology is unknown, but it may involve increased oxidative stress. A line of experimental evidence indicates that space flight may disrupt antioxidant defense system and result in increased oxidative stress. In vitro studies found that abundant of NO was produced in rat pheochromocytoma (PC12) cells, SHSY5Y neuroblastoma cells, and protein nitration was increased in PC12 cells within a simulated microgravity rotating wall bioreactor high aspect ratio vessel system or clinostat system. In the present study, we observed the change of redox status in SHSY5Y cells after parabolic flight, and studied the effects of key redox molecule, thioredoxin (TRX), during the altered gravity. SHSY5Y cells were divided into four groups: control cells, control cells transfected with TRX, flight cells and flight cells transfected with TRX. The expression levels of 3-nitrotyrosine (3-NT), inducible nitric oxide synthase (iNOS), TRX and thioredoxin reductase (TRXR) were observed by immunocytochemical method. It was shown that after parabolic flight, the staining of 3-NT and TRX were enhanced, while the expression level of TRXR was down-regulated compared with control. As for flight cells transfected with TRX, the staining of 3-NT and iNOS were weakened compared with flight cells. These results obtained suggest that altered gravity may increase protein nitration, down-regulate TRXR and elicit oxidative stress in SH-SY5Y cells, while TRX transfection could partly protect cells against oxidative stress induced by parabolic flight.

Key words: altered gravity; neuroblastoma; oxidative stress

Received: 2009-04-29　　Accepted: 2009-08-14

Corresponding author: 曲丽娜　　E-mail: linaqu@263.net

Citing This Article：

BI Lei, QU Li-Na, HUANG Zeng-Ming, WANG Chun-Yan, LI Qi, TAN Ying-Jun, LI Ying-Hui. [Effects of parabolic flight on redox status in SH-SY5Y cells.] [Ariticle in Chinese] . Acta Physiol Sin 2009; 61 (5): 445-450 (in Chinese with English abstract).