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#beta#_(2)--adrenoreceptor mediates the cardioprotection of ischemic preconditioning on myocardial contraction in rats subjected to ischemia/reperfusion injury

Wu Qin, Zhao Zhi, Sun Hong, Hao Yanling

Department of Physiology,Xuzhou Medical College.Xuzhou 221002,Jiangsu

Abstract

The presenr study aimed to investigate the role of #beta#_(2)-adrenoreceptor (#beta#_(2)-AR) in ischemic preconditioning (IP) in isolated rat heart model of ischemia/reperfusion (I/R). Sprague-Dawley rat hearts were quickly removed, mounted on Langendorff apparatus, and perfused with Krebs-Henseleit (KH) solution. After the initial stabilization period, the rats were randomly divided into 6 groups including control group (perfused for an additional 20 min), IP group (4 cycles of 5 min of ischemia followed by 5 min of reflow), isoproterenol (ISO) group (10 nmol/L of isoproterenol perfusion for 5 min followed by 5 min washout), IP + ICI118551 group (55 nmol/L ICI118551 perfusion for 5 min before and throughout IP), ISO + ICI118551 group (55 nmol/L ICI118551 perfusion for 5 min before and throughout ISO treatment), ICI118551 group (55 nmol/L ICI118551 perfusion for 20 min). After these treatments, all hearts were followed by 30 min no-flow ischemia and 30 min reperfusion. A computer-based electrophysiological recorder system was used to measure changes of the maximal rate of pressure increase in systole phase (+dp/dt_(max)), maximal rate of pressure decrease in diastole phase (-dp/dt_(max)), and difference of left ventricular pressure (#DELTA#LVP). Then cardiomyocytes from these hearts were isolated by 5 min of Ca~(2+)-free buffer perfusion and 25 min of collagenase perfusion. The ventricles were chopped and filtered. The myocytes were resuspended in KB buffer. The contraction and the viability of cardiomyocytes were measured. Lactate dehydrogenase (LDH) concentration in coronary effluent was assayed with assay kit. Both IP and ISO significantly increased the values of ±dp/dt_(max), #DELTA#LVP, the contraction and viability of cardiomyocytes, shortened the time-to-peak contraction (TTP), and decreased the release of LDH in coronary effluent. ICI118551, a selective #beta#_(2)-AR antagonist, blocked these effects. Either the time-to-50% relaxation (R_(50)) or the time-to-100% relaxation (R_(100)) had no significant differences in every group. Our results indicate that the cardioprotection of IP was mediated by #beta#_(2)-AR in isolated rat hearts subjected to I/R injury.

Key words: ischemic preconditioning;myocardial contraction;Adrenergic receptor

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Citing This Article：

Wu Qin, Zhao Zhi, Sun Hong, Hao Yanling. #beta#_(2)--adrenoreceptor mediates the cardioprotection of ischemic preconditioning on myocardial contraction in rats subjected to ischemia/reperfusion injury. Acta Physiol Sin 2008; 60 (3): 327-332 (in Chinese with English abstract).