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Changes in Bcl--2 and Caspase--3 expressions in cortex of hypoxic preconditioning mice

Zhang Yanbo, Lu Guowei, Yang Mingfeng, Niu Jingzhong, Sun Baoliang

Department of Neurology, Affiliated Hospital of Taishan Medical College.Tai'an 271000,Shandong；China

Abstract

The purpose of the present study was to explore the roles of Bcl-2 and Caspase-3 in mouse cortex in hypoxic preconditioning.Blb/c mice were randomly divided into three groups: control group, hypoxic group and hypoxic preconditioning group. Fluorescence intensity of Bcl-2 and Caspase-3 was observed and number of positive cells was counted in parietal cortex by immunofluorescence andconfocal laser scanning microscope. Fluorescence intensity of Bcl-2 in the normal group, hypoxic group and hypoxic preconditioning group was 6.2±1.7, 68.5±13.1,180.6±34.8, respectively, and number of Bcl-2-positive cells was 18.5±4.9, 52.3±10.5, 150.8±24.7,respectively. Fluorescence intensity of Caspase-3 in the control group, hypoxic group and hypoxic preconditioning group was 8.6±2.0, 40.2±8.2, 26.4±6.1, respectively, and number of Caspase-3-positive cells of was 4.3±1.2, 63.6±12.5, 45.7±9.8, respectively. Theresults showed that the expressions of Bcl-2 in both hypoxic group and hypoxic preconditioning group were significantly higher thanthat in the control group; and the expression of Bcl-2 in hypoxic preconditioning group was even higher than that in hypoxic group. The expressions of Caspase-3 in hypoxic group and hypoxic preconditioning group were also significantly higher than that in the control group; whereas the expression of Caspase-3 in hypoxic preconditioning group was significantly lower than that in hypoxic group.These results suggest that cortex cells are resistant to apoptosis via increased expression of Bcl-2 and lowered expression of Caspase-3 in the cortex and brain cells are thereby protected during hypoxic preconditioning.

Key words: Hypoxic preconditioning;Bcl-2;Caspase-3;Apoptosis;Cortex;Mice

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Citing This Article：

Zhang Yanbo, Lu Guowei, Yang Mingfeng, Niu Jingzhong, Sun Baoliang. Changes in Bcl--2 and Caspase--3 expressions in cortex of hypoxic preconditioning mice. Acta Physiol Sin 2008; 60 (2): 249-253 (in Chinese with English abstract).