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Analysis of high--frequency stimulation--evoked synaptic plasticity in mouse hippocampal CA1 region

Liu Xijuan, Huang Fensheng, Huang Chen, Yang Zhangmin, Feng Xinzheng

Department of Genetics and Molecular Biology, Medical School of Xi'an Jiaotong University/Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education.Xi'an 710061,Shaanxi；China；College of Life Science, Shaanxi Normal University.Xi'an 710062,Shaanxi

Abstract

Extracellular recordings of field excitatory postsynaptic potential (fEPSP) is one of the most common ways for studies of synaptic plasticity, such as long-term potentiation (LTP) and paired-pulse plasticity (PPP). The measurement of the changes in the different components of fEPSP waveform, such as initial slope, initial area, peak amplitude and whole area, were commonly used as criteria for the judgment of potentiation or depression of synaptic plasticity. However, the differences of the conclusions drawn from measuring different components of fEPSP waveform at the same recording have still been largely ignored. Here we compared high-frequency stimulation (HFS)-evoked synaptic plasticity, both LTP and PPP, by measuring different components of fEPSP waveform, including initial slope, initial area, peak amplitude, the whole area and the time courses. The results not only indicated the acceleration of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor kinetics underlies LTP in hippocampal CA1 region of mice, but also showed that different measurements of fEPSP waveform at the same recordings resulted in different magnitude of LTP and different forms of PPP in hippocampal CA1 region of mice, and some of them have drawn apparently contradictory conclusions.

Key words: analysis;Long-term potentiation;paired-pulse plasticity;Hippocampus;Mice

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Citing This Article：

Liu Xijuan, Huang Fensheng, Huang Chen, Yang Zhangmin, Feng Xinzheng. Analysis of high--frequency stimulation--evoked synaptic plasticity in mouse hippocampal CA1 region. Acta Physiol Sin 2008; 60 (2): 284-291 (in Chinese with English abstract).