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[Effect of pulse magnetic field on distribution of neuronal action potential.] [Article in Chinese]

ZHENG Yu, CAI Di, WANG Jin-Hai, LI Gang, LIN Ling

School of Electronics and Information Engineering, Tianjin Polytechnic University, Tianjin 300387, China； State Key Laboratory of Precision Measurement Technology and Instruments, Tianjin University, Tianjin 300072, China

Abstract

The biological effect on the organism generated by magnetic field is widely studied. The present study was aimed to observe the change of sodium channel under magnetic field in neurons. Cortical neurons of Kunming mice were isolated, subjected to 15 Hz, 1 mT pulse magnetic stimulation, and then the currents of neurons were recorded by whole-cell patch clamp. The results showed that, under magnetic stimulation, the activation process of Na⁺ channel was delayed, and the inactivation process was accelerated. Given the classic three-layer model, the polarization diagram of cell membrane potential distribution under pulse magnetic field was simulated, and it was found that the membrane potential induced was associated with the frequency and intensity of magnetic field. Also the effect of magnetic field-induced current on action potential was simulated by Hodgkin-Huxley (H-H) model. The result showed that the generation of action potential was delayed, and frequency and the amplitudes were decreased when working current was between -1.32 μA and 0 μA. When the working current was higher than 0 μA, the generation frequency of action potential was increased, and the change of amplitudes was not obvious, and when the working current was lower than -1.32 μA, the time of rising edge and amplitudes of action potential were decreased drastically, and the action potential was unable to generate. These results suggest that the magnetic field simulation can affect the distribution frequency and amplitude of action potential of neuron via sodium channel mediation.

Key words: Transmembrane potential; action potential; sodium ion channel; distribution characteristics

Received: 2013-12-02　　Accepted: 2014-02-13

Corresponding author: 王金海　　E-mail: wangjinhai@tjpu.edu.cn

Citing This Article：

ZHENG Yu, CAI Di, WANG Jin-Hai, LI Gang, LIN Ling. [Effect of pulse magnetic field on distribution of neuronal action potential.] [Article in Chinese]. Acta Physiol Sin 2014; 66 (4): 438-448 (in Chinese with English abstract).