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Relationship between hydrogen sulfide and myocardial damage in endotoxemic rats

Xian Xiaohui, Huang Xinli, Zhou Xiaohong, Zhang Jingkun, Ling Yiling

Department of Pathophysiology, Hebei Medical University.Shijiazhuang 050017,Hebei

Abstract

To investigate the changes and role of hydrogen sulfide （H_(2)S） in myocardial damage in endotoxemic rats, a rat model of endotoxemia induced by injection of lipopolysaccharide （LPS） was developed. Male Wistar rats were divided into four groups： control group, LPS group, LPS + propargylglycine （PPG, a metabolic enzyme inhibitor of H_(2)S） group and LPS + NariS （H_(2)S donor） group. The mean arterial pressure （MAP） of rats within 4 h was observed, TNF-#alpha# and H_(2)S contents in plasma, TNF-#alpha# and H_(2)S contents, lactate dehydrogenase （LDH） and myeloperoxidase （MPO） activity in cardiac muscles were determined. The morphological structure of cardiac muscle was observed. Administration of LPS caused a sustained fall in MAP within 4 h, and significant increases in TNF-#alpha# and H_(2)S contents in plasma （P〈0.05）. Plasmic H_(2)S content was negatively correlated with MAP （r = -0.936, -0.913 and -0.908 at 1, 2 and 4 h, respectively, P〈0.05）. LPS also induced increases in TNF-#alpha# and H_(2)S contents, LDH and MPO activity in cardiac muscles and myocardial damage. Treatment with PPG reduced the increases in TNF-#alpha# and H_(2)S contents in plasma, TNF-#alpha# and H_(2)S contents, LDH and MPO activity in cardiac muscles, ameliorated the hypotensive effect and myocardial damage caused by LPS administration （P〈 0.05）. However, treatment with NaHS increased TNF-#alpha# and H_(2)S contents in plasma, TNF-#alpha# and H_(2)S contents, LDH and MPO activity in cardiac muscles, and aggravated the hypotensive action and tissue injuries caused by LPS administration （P〈0.05）. It issuggested that hypotension and myocardial damage in endotoxemic rats are partly induced by increase in H_(2)S content.

Key words: hydrogen sulfide;Endotoxin;Cardiac muscle;Rat

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Citing This Article：

Xian Xiaohui, Huang Xinli, Zhou Xiaohong, Zhang Jingkun, Ling Yiling. Relationship between hydrogen sulfide and myocardial damage in endotoxemic rats. Acta Physiol Sin 2007; 59 (3): 357-362 (in Chinese with English abstract).