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Differential analysis of proteomic profiles between cryptorchid and normal mouse testes

Li Enzhong, Li Dexue, Zhang Shiqing, Li Lan, Wang Changyong, Zhang Xueming, Lu Jingyan, Liu Yikai

Institute of Basic Medical Sciences, Academy of Military Medical Science.Beijing 100850；China

Abstract

To screen factors related to spermatogonial stem cell （SSC） proliferation, and to investigate the mechanism of infertility caused by cryptorchidism, ten-day-old Kunming （KM） mice were used and experimental cryptorchidism was conducted. On the 35th day after cryptorchid operation, the left testes were fixed in Bouin's fluid and used for histological analysis. The testes of 45-day-old mice were subjected to the same histological analysis, and it was found that they contained germ cells at every stage of development, from SSCs to sperm, indicating that the animals were fully sexually mature at this age. While in experimental cryptorchid mice, the spermatogenesis was arrested at the stage of spermatocytes, and only spermatogonia and primary spermatocytes were present in cryptorchid testes. The proportion of spermatogonia to other types of germ cells was much higher than that in sexually mature mice. On the other hand, the fight testes were used for proteomic analysis. The total protein in testes was extracted on the 35th day after cryptorchid operation. The differentially expressed proteins in cryptorchid mice and sexually mature mice were screened and compared by the proteomic techniques. Through the separation of two-dimensional gel electrophoresis （2-DE）, 20 differential protein spots were found, and 9 of them were digested and identified by the matrix-assisted laser desorption/ionization-time of flight （MALDI-TOF） mass spectrum. In cryptorchid mice, 6 out of 9 proteins were down-regulated, and 3 were up-regulated. Among these proteins, 4 proteins were identified, and they were Stathmin, phosphatidylethanolamine-binding protein1 （PEBP1）, HES-related basic helix-loop-helixprotein （HERP）, and one unnamed protein （we temporarily named it Px）. More Stathmin, PEBP 1 and Px were expressed in sexually mature mice than in experimental cryptorchid mice. But HERP1 was the other way round. In the present study, we have screened 4 proteins related to cryptorchidism. It is helpful to study the mechanism of SSC proliferation and infertility caused by cryptorchidism.

Key words: cryptorchidism;Mice;protein expression profile

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Citing This Article：

Li Enzhong, Li Dexue, Zhang Shiqing, Li Lan, Wang Changyong, Zhang Xueming, Lu Jingyan, Liu Yikai. Differential analysis of proteomic profiles between cryptorchid and normal mouse testes. Acta Physiol Sin 2007; 59 (3): 345-350 (in Chinese with English abstract).